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Potassium Channels

Overview of attention for book
Cover of 'Potassium Channels'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation
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    Chapter 2 Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome Using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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    Chapter 3 Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane Using Surface Biotinylation
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    Chapter 4 Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome
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    Chapter 5 Probing Subunits Interactions in KATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine
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    Chapter 6 Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization
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    Chapter 7 Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function
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    Chapter 8 Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel Mutations
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    Chapter 9 Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters
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    Chapter 10 Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions
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    Chapter 11 Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes
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    Chapter 12 Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations
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    Chapter 13 Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET
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    Chapter 14 Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters
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    Chapter 15 Combinatorial Assembly of Lumitoxins
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    Chapter 16 Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay
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    Chapter 17 Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies
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    Chapter 18 In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish
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    Chapter 19 Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes
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    Chapter 20 Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels
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    Chapter 21 CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion Channels
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    Chapter 22 Ion Binding to Transport Proteins using Isothermal Titration Calorimetry
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    Chapter 23 Building Atomic Models of the Ion Channels Based on Low Resolution Electron Microscopy Maps and Homology Modeling
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    Chapter 24 Studying Kv Channels Function using Computational Methods
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    Chapter 25 Erratum to: Ion Binding to Transport Proteins using Isothermal Titration Calorimetry
Attention for Chapter 7: Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function
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Chapter title
Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function
Chapter number 7
Book title
Potassium Channels
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7362-0_7
Pubmed ID
Book ISBNs
978-1-4939-7361-3, 978-1-4939-7362-0
Authors

Nicholas C. Vierra, Matthew T. Dickerson, Louis H. Philipson, David A. Jacobson

Abstract

Stimulus-secretion coupling in pancreatic β-cells requires Ca(2+) influx through voltage-dependent Ca(2+) channels, whose activity is controlled by the plasma membrane potential (V m). Here, we present a method of measuring fluctuations in the β-cell V m and Ca(2+) influx simultaneously, which provides valuable information about the ionic signaling mechanisms that underlie insulin secretion. This chapter describes the use of perforated patch clamp electrophysiology on cells loaded with a fluorescent intracellular Ca(2+) indicator, which permits the stable recording conditions needed to monitor the V m and Ca(2+) influx in β-cells. Moreover, this chapter describes the protocols necessary for the preparation of mouse and human islet cells for the simultaneous recording of V m and Ca(2+) as well as determining the specific islet cell type assessed in each experiment.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 20%
Student > Ph. D. Student 1 20%
Other 1 20%
Student > Postgraduate 1 20%
Unknown 1 20%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%
Unknown 3 60%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2018.
All research outputs
#18,574,814
of 23,006,268 outputs
Outputs from Methods in molecular biology
#7,961
of 13,160 outputs
Outputs of similar age
#330,447
of 442,258 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,498 outputs
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So far Altmetric has tracked 13,160 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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