Chapter title |
Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function
|
---|---|
Chapter number | 7 |
Book title |
Potassium Channels
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7362-0_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7361-3, 978-1-4939-7362-0
|
Authors |
Nicholas C. Vierra, Matthew T. Dickerson, Louis H. Philipson, David A. Jacobson |
Abstract |
Stimulus-secretion coupling in pancreatic β-cells requires Ca(2+) influx through voltage-dependent Ca(2+) channels, whose activity is controlled by the plasma membrane potential (V m). Here, we present a method of measuring fluctuations in the β-cell V m and Ca(2+) influx simultaneously, which provides valuable information about the ionic signaling mechanisms that underlie insulin secretion. This chapter describes the use of perforated patch clamp electrophysiology on cells loaded with a fluorescent intracellular Ca(2+) indicator, which permits the stable recording conditions needed to monitor the V m and Ca(2+) influx in β-cells. Moreover, this chapter describes the protocols necessary for the preparation of mouse and human islet cells for the simultaneous recording of V m and Ca(2+) as well as determining the specific islet cell type assessed in each experiment. |
X Demographics
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United States | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 5 | 100% |
Demographic breakdown
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Student > Bachelor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Other | 1 | 20% |
Student > Postgraduate | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Unknown | 3 | 60% |