↓ Skip to main content

Potassium Channels

Overview of attention for book
Cover of 'Potassium Channels'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation
  3. Altmetric Badge
    Chapter 2 Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome Using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes
  4. Altmetric Badge
    Chapter 3 Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane Using Surface Biotinylation
  5. Altmetric Badge
    Chapter 4 Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome
  6. Altmetric Badge
    Chapter 5 Probing Subunits Interactions in KATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine
  7. Altmetric Badge
    Chapter 6 Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization
  8. Altmetric Badge
    Chapter 7 Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function
  9. Altmetric Badge
    Chapter 8 Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel Mutations
  10. Altmetric Badge
    Chapter 9 Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters
  11. Altmetric Badge
    Chapter 10 Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions
  12. Altmetric Badge
    Chapter 11 Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes
  13. Altmetric Badge
    Chapter 12 Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations
  14. Altmetric Badge
    Chapter 13 Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET
  15. Altmetric Badge
    Chapter 14 Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters
  16. Altmetric Badge
    Chapter 15 Combinatorial Assembly of Lumitoxins
  17. Altmetric Badge
    Chapter 16 Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay
  18. Altmetric Badge
    Chapter 17 Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies
  19. Altmetric Badge
    Chapter 18 In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish
  20. Altmetric Badge
    Chapter 19 Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes
  21. Altmetric Badge
    Chapter 20 Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels
  22. Altmetric Badge
    Chapter 21 CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion Channels
  23. Altmetric Badge
    Chapter 22 Ion Binding to Transport Proteins using Isothermal Titration Calorimetry
  24. Altmetric Badge
    Chapter 23 Building Atomic Models of the Ion Channels Based on Low Resolution Electron Microscopy Maps and Homology Modeling
  25. Altmetric Badge
    Chapter 24 Studying Kv Channels Function using Computational Methods
  26. Altmetric Badge
    Chapter 25 Erratum to: Ion Binding to Transport Proteins using Isothermal Titration Calorimetry
Attention for Chapter 5: Probing Subunits Interactions in KATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine
Altmetric Badge

Readers on

mendeley
2 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Probing Subunits Interactions in KATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine
Chapter number 5
Book title
Potassium Channels
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7362-0_5
Pubmed ID
Book ISBNs
978-1-4939-7361-3, 978-1-4939-7362-0
Authors

Prasanna Devaraneni, Emily A. Rex, Show-Ling Shyng

Abstract

Potassium channels are multimeric protein complexes regulated by diverse physiological and pharmacological ligands. The key to understanding mechanisms of channel regulation is the ability to detect structural changes associated with ligand binding. While high-resolution structural methods such as X-ray crystallography and single-particle cryo-electron microscopy offer direct visualization of channel structures, these methods do have limitations and may not be suitable for the question of interest. In this chapter, we describe the use of a photo-cross-linker unnatural amino acid, p-azido-L-phenylalanine, to probe interactions between two proteins, the sulfonylurea receptor 1 and the inwardly rectifying potassium channel Kir6.2, that form the ATP-sensitive potassium (KATP) channel complex in the absence or presence of ligands. The difference in the extent of crosslinking between a liganded state and unliganded state can be used as a readout of ligand-induced structural changes. We anticipate that the protocol described here will also be applicable for other potassium channels and protein complexes.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Student > Bachelor 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Chemistry 1 50%