Chapter title |
Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters
|
---|---|
Chapter number | 9 |
Book title |
Potassium Channels
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7362-0_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7361-3, 978-1-4939-7362-0
|
Authors |
C. David Weaver, Weaver, C. David |
Abstract |
Monovalent cation channels are critically important for physiological processes ranging from the control of neuronal excitability to the maintenance of solute balance. Mutations in these channels are associated with a multiplicity of diseases and monovalent cation channel-modulating drugs are used as therapeutics. Techniques that allow the measurement of the activity of these ion channels are useful for exploring their many biological roles as well as enabling the discovery and characterization of ion channel modulators for the purposes of drug discovery. Although there are numerous techniques for measuring the activity of monovalent cation channels, the thallium flux assay technique is a widely used fluorescence-based approach. Described herein is a method for using the thallium-flux technique for detecting and quantifying the activity of small-molecule potassium channel modulators in 384-well plates. |
X Demographics
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United States | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 17 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 6 | 35% |
Researcher | 2 | 12% |
Student > Doctoral Student | 1 | 6% |
Unspecified | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 29% |
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Pharmacology, Toxicology and Pharmaceutical Science | 3 | 18% |
Unspecified | 1 | 6% |
Chemical Engineering | 1 | 6% |
Other | 0 | 0% |
Unknown | 5 | 29% |