Chapter title |
In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish
|
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Chapter number | 18 |
Book title |
Potassium Channels
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7362-0_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7361-3, 978-1-4939-7362-0
|
Authors |
Jui-Yi Hsieh, Diane M. Papazian |
Abstract |
Zebrafish is a lower vertebrate model organism that facilitates integrative analysis of the in vivo effects of potassium and other ion channel mutations at the molecular, cellular, developmental, circuit, systems, and behavioral levels of analysis. Here, we describe a method for extracellular, loose patch electrophysiological recording of electrical activity in cerebellar Purkinje cells in living, awake zebrafish, with the goal of investigating pathological mechanisms underlying channelopathies or other diseases that disrupt cerebellar function. Purkinje cell excitability and a functional cerebellar circuit develop rapidly in zebrafish and show strong conservation with the mammalian cerebellum. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Lecturer > Senior Lecturer | 2 | 33% |
Student > Ph. D. Student | 1 | 17% |
Researcher | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Student > Master | 1 | 17% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 33% |
Neuroscience | 2 | 33% |
Psychology | 1 | 17% |
Unknown | 1 | 17% |