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Potassium Channels

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Cover of 'Potassium Channels'

Table of Contents

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    Book Overview
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    Chapter 1 Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation
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    Chapter 2 Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome Using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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    Chapter 3 Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane Using Surface Biotinylation
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    Chapter 4 Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome
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    Chapter 5 Probing Subunits Interactions in KATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine
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    Chapter 6 Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization
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    Chapter 7 Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function
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    Chapter 8 Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel Mutations
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    Chapter 9 Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters
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    Chapter 10 Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions
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    Chapter 11 Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes
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    Chapter 12 Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations
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    Chapter 13 Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET
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    Chapter 14 Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters
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    Chapter 15 Combinatorial Assembly of Lumitoxins
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    Chapter 16 Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay
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    Chapter 17 Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies
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    Chapter 18 In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish
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    Chapter 19 Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes
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    Chapter 20 Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels
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    Chapter 21 CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion Channels
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    Chapter 22 Ion Binding to Transport Proteins using Isothermal Titration Calorimetry
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    Chapter 23 Building Atomic Models of the Ion Channels Based on Low Resolution Electron Microscopy Maps and Homology Modeling
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    Chapter 24 Studying Kv Channels Function using Computational Methods
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    Chapter 25 Erratum to: Ion Binding to Transport Proteins using Isothermal Titration Calorimetry
Attention for Chapter 16: Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay
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Chapter title
Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay
Chapter number 16
Book title
Potassium Channels
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7362-0_16
Pubmed ID
Book ISBNs
978-1-4939-7361-3, 978-1-4939-7362-0
Authors

Michael J. Litt, Roger D. Cone, Masoud Ghamari-Langroudi

Abstract

The family of inward rectifying potassium channels (Kir channels) plays crucial roles in the regulation of heart rhythms, renal excretion, insulin release, and neuronal activity. Their dysfunction has been attributed to numerous diseases such as cardiac arrhythmia, kidney failure and electrolyte imbalance, diabetes mellitus, epilepsy, retinal degeneration, and other neuronal disorders. We have recently demonstrated that the melanocortin-4 receptor (MC4R), a Gαs-coupled GPCR, regulates Kir7.1 activity through a mechanism independent of Gαs and cAMP. In contrast to the many other members of the Kir channel family, less is known about the biophysical properties, regulation, and physiological functions of Kir7.1. In addition to using conventional patch clamp techniques, we have employed a high-throughput Tl(+) flux assay to further investigate the kinetics of MC4R-Kir7.1 signaling in vitro. Here, we discuss the employment of the Tl(+) flux assay to study MC4R -mediated regulation of Kir7.1 activity and to screen compounds for drug discovery.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 20%
Professor 1 20%
Student > Bachelor 1 20%
Unknown 2 40%
Readers by discipline Count As %
Neuroscience 1 20%
Materials Science 1 20%
Medicine and Dentistry 1 20%
Unknown 2 40%