Chapter title |
Technical Review: Microscopy and Image Processing Tools to Analyze Plant Chromatin: Practical Considerations
|
---|---|
Chapter number | 31 |
Book title |
Plant Chromatin Dynamics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7318-7_31 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7317-0, 978-1-4939-7318-7
|
Authors |
Célia Baroux, Veit Schubert, Baroux, Célia, Schubert, Veit |
Abstract |
In situ nucleus and chromatin analyses rely on microscopy imaging that benefits from versatile, efficient fluorescent probes and proteins for static or live imaging. Yet the broad choice in imaging instruments offered to the user poses orientation problems. Which imaging instrument should be used for which purpose? What are the main caveats and what are the considerations to best exploit each instrument's ability to obtain informative and high-quality images? How to infer quantitative information on chromatin or nuclear organization from microscopy images? In this review, we present an overview of common, fluorescence-based microscopy systems and discuss recently developed super-resolution microscopy systems, which are able to bridge the resolution gap between common fluorescence microscopy and electron microscopy. We briefly present their basic principles and discuss their possible applications in the field, while providing experience-based recommendations to guide the user toward best-possible imaging. In addition to raw data acquisition methods, we discuss commercial and noncommercial processing tools required for optimal image presentation and signal evaluation in two and three dimensions. |
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Unknown | 2 | 100% |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 15 | 100% |
Demographic breakdown
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Researcher | 5 | 33% |
Unspecified | 1 | 7% |
Other | 1 | 7% |
Student > Bachelor | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Other | 2 | 13% |
Unknown | 4 | 27% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 20% |
Agricultural and Biological Sciences | 3 | 20% |
Unspecified | 1 | 7% |
Computer Science | 1 | 7% |
Physics and Astronomy | 1 | 7% |
Other | 1 | 7% |
Unknown | 5 | 33% |