Report for: Plant Chromatin Dynamics

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Chapter title	In Vitro Assays to Measure Histone Methyltransferase Activity Using Different Chromatin Substrates
Chapter number	20
Book title	Plant Chromatin Dynamics
Published in	Methods in molecular biology, January 2018
DOI	10.1007/978-1-4939-7318-7_20
Pubmed ID	29052201
Book ISBNs	978-1-4939-7317-0, 978-1-4939-7318-7
Authors	Yannick Jacob, Philipp Voigt
Abstract	In vitro histone modification (HM) assays are used to characterize the activity of chromatin-modifying enzymes. These assays provide information regarding the modification sites on histones, the product specificity, and the impact of other histone or nucleotide modifications on enzyme activity. In particular, histone methyltransferase (HMT) assays have been instrumental in elucidating the activity and site specificity of many plant HMT enzymes. In this chapter, we describe a general protocol that can be used to perform HMT assays using different chromatin substrates, detection methods, and enzymes directly purified from plant material or heterologous sources.

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Geographical breakdown

Country	Count	As %
Unknown	1	100%

Demographic breakdown

Type	Count	As %
Members of the public	1	100%

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	6	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Ph. D. Student	2	33%
Researcher	1	17%
Student > Master	1	17%
Unknown	2	33%

Readers by discipline	Count	As %
Biochemistry, Genetics and Molecular Biology	3	50%
Agricultural and Biological Sciences	2	33%
Unknown	1	17%

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Plant Chromatin Dynamics

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