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Therapeutic Oligonucleotides

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Cover of 'Therapeutic Oligonucleotides'

Table of Contents

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    Book Overview
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    Chapter 1 Therapeutic oligonucleotides.
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    Chapter 2 Therapeutic Oligonucleotides
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    Chapter 3 2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNACOC)
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    Chapter 4 A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery.
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    Chapter 5 Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides.
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    Chapter 6 A nanoparticle for tumor targeted delivery of oligomers.
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    Chapter 7 Light-Directed Delivery of Nucleic Acids
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    Chapter 8 Antibody Targeted siRNA Delivery.
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    Chapter 9 Aptamer-drug conjugation for targeted tumor cell therapy.
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    Chapter 10 Five-Step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
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    Chapter 11 Diverse Small Non-coding RNAs in RNA Interference Pathways.
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    Chapter 12 Quantification of siRNAs In Vitro and In Vivo.
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    Chapter 13 Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.
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    Chapter 14 siRNA Knockdown of Gene Expression in Endothelial Cells.
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    Chapter 15 Using RNA Interference in Schistosoma mansoni
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    Chapter 16 Performing the Labeled microRNA Pull-Down (LAMP) Assay System: An Experimental Approach for High-Throughput Identification of microRNA-Target mRNAs.
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    Chapter 17 Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8
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    Chapter 18 Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-Like Receptor 9
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    Chapter 19 Surface Plasmon Resonance Investigation of RNA Aptamer–RNA Ligand Interactions
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    Chapter 20 Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein
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    Chapter 21 Inhibition of Human Papillomavirus Expression Using DNAzymes
Attention for Chapter 4: A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery.
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Chapter title
A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery.
Chapter number 4
Book title
Therapeutic Oligonucleotides
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-188-8_4
Pubmed ID
Book ISBNs
978-1-61779-187-1, 978-1-61779-188-8
Authors

Laurence Crombez, May C. Morris, Frederic Heitz, Gilles Divita

Abstract

The dramatic acceleration in identification of new nucleic acid-based therapeutic molecules such as short interfering RNA (siRNA) and peptide-nucleic acid (PNA) analogues has provided new perspectives for therapeutic targeting of specific genes responsible for pathological disorders. However, the poor cellular uptake of nucleic acids together with the low permeability of the cell membrane to negatively charged molecules remain major obstacles to their clinical development. Several non-viral strategies have been proposed to improve the delivery of synthetic short oligonucleotides both in cultured cells and in vivo. Cell-penetrating peptides constitute very promising tools for non-invasive cellular import of oligonucleotides and analogs. We recently described a non-covalent strategy based on short amphiphatic peptides (MPG8/PEP3) that have been successfully applied ex vivo and in vivo for the delivery of therapeutic siRNA and PNA molecules. PEP3 and MPG8 form stable nanoparticles with PNA analogues and siRNA, respectively, and promote their efficient cellular uptake, independently of the endosomal pathway, into a wide variety of cell lines, including primary and suspension lines, without any associated cytotoxicity. This chapter describes easy-to-handle protocols for the use of MPG-8 or PEP-3-nanoparticle technologies for PNA and siRNA delivery into adherent and suspension cell lines as well as in vivo into cancer mouse models.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 5%
Unknown 18 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 26%
Student > Master 4 21%
Professor > Associate Professor 3 16%
Researcher 3 16%
Other 1 5%
Other 2 11%
Unknown 1 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 32%
Biochemistry, Genetics and Molecular Biology 5 26%
Chemistry 2 11%
Computer Science 1 5%
Psychology 1 5%
Other 1 5%
Unknown 3 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2011.
All research outputs
#3,108,458
of 4,506,214 outputs
Outputs from Methods in molecular biology
#1,457
of 3,194 outputs
Outputs of similar age
#36,311
of 54,625 outputs
Outputs of similar age from Methods in molecular biology
#8
of 9 outputs
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So far Altmetric has tracked 3,194 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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