↓ Skip to main content

Therapeutic Oligonucleotides

Overview of attention for book
Cover of 'Therapeutic Oligonucleotides'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Therapeutic oligonucleotides.
  3. Altmetric Badge
    Chapter 2 Therapeutic Oligonucleotides
  4. Altmetric Badge
    Chapter 3 2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNACOC)
  5. Altmetric Badge
    Chapter 4 A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery.
  6. Altmetric Badge
    Chapter 5 Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides.
  7. Altmetric Badge
    Chapter 6 A nanoparticle for tumor targeted delivery of oligomers.
  8. Altmetric Badge
    Chapter 7 Light-Directed Delivery of Nucleic Acids
  9. Altmetric Badge
    Chapter 8 Antibody Targeted siRNA Delivery.
  10. Altmetric Badge
    Chapter 9 Aptamer-drug conjugation for targeted tumor cell therapy.
  11. Altmetric Badge
    Chapter 10 Five-Step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
  12. Altmetric Badge
    Chapter 11 Diverse Small Non-coding RNAs in RNA Interference Pathways.
  13. Altmetric Badge
    Chapter 12 Quantification of siRNAs In Vitro and In Vivo.
  14. Altmetric Badge
    Chapter 13 Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.
  15. Altmetric Badge
    Chapter 14 siRNA Knockdown of Gene Expression in Endothelial Cells.
  16. Altmetric Badge
    Chapter 15 Using RNA Interference in Schistosoma mansoni
  17. Altmetric Badge
    Chapter 16 Performing the Labeled microRNA Pull-Down (LAMP) Assay System: An Experimental Approach for High-Throughput Identification of microRNA-Target mRNAs.
  18. Altmetric Badge
    Chapter 17 Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8
  19. Altmetric Badge
    Chapter 18 Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-Like Receptor 9
  20. Altmetric Badge
    Chapter 19 Surface Plasmon Resonance Investigation of RNA Aptamer–RNA Ligand Interactions
  21. Altmetric Badge
    Chapter 20 Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein
  22. Altmetric Badge
    Chapter 21 Inhibition of Human Papillomavirus Expression Using DNAzymes
Attention for Chapter 12: Quantification of siRNAs In Vitro and In Vivo.
Altmetric Badge

Mentioned by

twitter
1 tweeter

Citations

dimensions_citation
6 Dimensions

Readers on

mendeley
17 Mendeley
citeulike
1 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Quantification of siRNAs In Vitro and In Vivo.
Chapter number 12
Book title
Therapeutic Oligonucleotides
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-188-8_12
Pubmed ID
Book ISBNs
978-1-61779-187-1, 978-1-61779-188-8
Authors

Angie Cheng, Alexander V. Vlassov, Susan Magdaleno

Abstract

RNA interference (RNAi) is a regulatory mechanism of eukaryotic cells that uses small interfering RNAs (siRNA) to direct homology-dependent control of gene activity. Applications of RNAi include functional genomics, in vivo target validation, and gene-specific medicines. A key to in vivo application of siRNA is the advancement of efficient delivery to organs, tissues, or cell types of interest. There is a need to develop reliable and easy-to-use assays to evaluate siRNA delivery efficiency and distribution, study pathways, and stability of siRNAs in cells (post-transfection) and in animals (post- injection). We have adopted the Applied Biosystems TaqMan(®) based stem-loop RT-PCR technology, originally developed for quantification of endogenous microRNAs in cells, to fulfill these needs. In this chapter, application protocols are described, which enable robust quantification of siRNA, including chemically modified molecules, in vitro and in vivo.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 47%
Researcher 4 24%
Professor 2 12%
Student > Doctoral Student 2 12%
Student > Postgraduate 1 6%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 65%
Pharmacology, Toxicology and Pharmaceutical Science 2 12%
Chemistry 2 12%
Medicine and Dentistry 2 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2011.
All research outputs
#3,108,431
of 4,506,407 outputs
Outputs from Methods in molecular biology
#1,460
of 3,194 outputs
Outputs of similar age
#36,522
of 54,625 outputs
Outputs of similar age from Methods in molecular biology
#8
of 9 outputs
Altmetric has tracked 4,506,407 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,194 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 54,625 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one.