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Therapeutic Oligonucleotides

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Cover of 'Therapeutic Oligonucleotides'

Table of Contents

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    Book Overview
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    Chapter 1 Therapeutic oligonucleotides.
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    Chapter 2 Therapeutic Oligonucleotides
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    Chapter 3 2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNACOC)
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    Chapter 4 A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery.
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    Chapter 5 Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides.
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    Chapter 6 A nanoparticle for tumor targeted delivery of oligomers.
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    Chapter 7 Light-Directed Delivery of Nucleic Acids
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    Chapter 8 Antibody Targeted siRNA Delivery.
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    Chapter 9 Aptamer-drug conjugation for targeted tumor cell therapy.
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    Chapter 10 Five-Step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
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    Chapter 11 Diverse Small Non-coding RNAs in RNA Interference Pathways.
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    Chapter 12 Quantification of siRNAs In Vitro and In Vivo.
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    Chapter 13 Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.
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    Chapter 14 siRNA Knockdown of Gene Expression in Endothelial Cells.
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    Chapter 15 Using RNA Interference in Schistosoma mansoni
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    Chapter 16 Performing the Labeled microRNA Pull-Down (LAMP) Assay System: An Experimental Approach for High-Throughput Identification of microRNA-Target mRNAs.
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    Chapter 17 Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8
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    Chapter 18 Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-Like Receptor 9
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    Chapter 19 Surface Plasmon Resonance Investigation of RNA Aptamer–RNA Ligand Interactions
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    Chapter 20 Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein
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    Chapter 21 Inhibition of Human Papillomavirus Expression Using DNAzymes
Attention for Chapter 12: Quantification of siRNAs In Vitro and In Vivo.
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Chapter title
Quantification of siRNAs In Vitro and In Vivo.
Chapter number 12
Book title
Therapeutic Oligonucleotides
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-188-8_12
Pubmed ID
21748641
Book ISBNs
978-1-61779-187-1, 978-1-61779-188-8
Authors

Angie Cheng, Alexander V. Vlassov, Susan Magdaleno

Abstract

RNA interference (RNAi) is a regulatory mechanism of eukaryotic cells that uses small interfering RNAs (siRNA) to direct homology-dependent control of gene activity. Applications of RNAi include functional genomics, in vivo target validation, and gene-specific medicines. A key to in vivo application of siRNA is the advancement of efficient delivery to organs, tissues, or cell types of interest. There is a need to develop reliable and easy-to-use assays to evaluate siRNA delivery efficiency and distribution, study pathways, and stability of siRNAs in cells (post-transfection) and in animals (post- injection). We have adopted the Applied Biosystems TaqMan(®) based stem-loop RT-PCR technology, originally developed for quantification of endogenous microRNAs in cells, to fulfill these needs. In this chapter, application protocols are described, which enable robust quantification of siRNA, including chemically modified molecules, in vitro and in vivo.

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Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 47%
Researcher 4 24%
Professor 2 12%
Student > Doctoral Student 2 12%
Student > Postgraduate 1 6%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 65%
Pharmacology, Toxicology and Pharmaceutical Science 2 12%
Chemistry 2 12%
Medicine and Dentistry 2 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2011.
All research outputs
#3,108,431
of 4,506,407 outputs
Outputs from Methods in molecular biology
#1,460
of 3,194 outputs
Outputs of similar age
#36,522
of 54,625 outputs
Outputs of similar age from Methods in molecular biology
#8
of 9 outputs
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So far Altmetric has tracked 3,194 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 54,625 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one.

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