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Therapeutic Oligonucleotides

Overview of attention for book
Cover of 'Therapeutic Oligonucleotides'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Therapeutic oligonucleotides.
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    Chapter 2 Therapeutic Oligonucleotides
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    Chapter 3 2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNACOC)
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    Chapter 4 A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery.
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    Chapter 5 Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides.
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    Chapter 6 A nanoparticle for tumor targeted delivery of oligomers.
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    Chapter 7 Light-Directed Delivery of Nucleic Acids
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    Chapter 8 Antibody Targeted siRNA Delivery.
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    Chapter 9 Aptamer-drug conjugation for targeted tumor cell therapy.
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    Chapter 10 Five-Step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
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    Chapter 11 Diverse Small Non-coding RNAs in RNA Interference Pathways.
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    Chapter 12 Quantification of siRNAs In Vitro and In Vivo.
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    Chapter 13 Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.
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    Chapter 14 siRNA Knockdown of Gene Expression in Endothelial Cells.
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    Chapter 15 Using RNA Interference in Schistosoma mansoni
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    Chapter 16 Performing the Labeled microRNA Pull-Down (LAMP) Assay System: An Experimental Approach for High-Throughput Identification of microRNA-Target mRNAs.
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    Chapter 17 Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8
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    Chapter 18 Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-Like Receptor 9
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    Chapter 19 Surface Plasmon Resonance Investigation of RNA Aptamer–RNA Ligand Interactions
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    Chapter 20 Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein
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    Chapter 21 Inhibition of Human Papillomavirus Expression Using DNAzymes
Attention for Chapter 14: siRNA Knockdown of Gene Expression in Endothelial Cells.
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Chapter title
siRNA Knockdown of Gene Expression in Endothelial Cells.
Chapter number 14
Book title
Therapeutic Oligonucleotides
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-188-8_14
Pubmed ID
Book ISBNs
978-1-61779-187-1, 978-1-61779-188-8
Authors

Emily Dennstedt, Brad Bryan

Abstract

Cultured endothelial cells are renowned for being difficult to transfect, whether for the purpose of exogenous over-expression of plasmid DNA or for genetic knockdown via silencing RNA. Therefore, optimal conditions are absolutely necessary for achieving relatively high transfection efficiency coupled with low cellular toxicity in endothelial cells. This chapter will detail an optimized protocol that has been shown to knockdown gene expression using siRNA in primary cultures of human umbilical vein endothelial cells (HUVECs) - perhaps the most widely utilized endothelial cell line for vascular research. While developed for optimal siRNA transfection of HUVECs, aspects of this protocol can be empirically modified to yield efficient siRNA transfection in most other cell lines.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
United States 1 2%
Taiwan 1 2%
Unknown 42 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 20%
Researcher 7 16%
Student > Master 7 16%
Student > Bachelor 7 16%
Student > Postgraduate 4 9%
Other 6 13%
Unknown 5 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 24%
Agricultural and Biological Sciences 11 24%
Chemistry 4 9%
Pharmacology, Toxicology and Pharmaceutical Science 3 7%
Medicine and Dentistry 3 7%
Other 6 13%
Unknown 7 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2011.
All research outputs
#3,108,588
of 4,506,407 outputs
Outputs from Methods in molecular biology
#1,460
of 3,194 outputs
Outputs of similar age
#36,699
of 54,728 outputs
Outputs of similar age from Methods in molecular biology
#8
of 9 outputs
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So far Altmetric has tracked 3,194 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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