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Therapeutic Oligonucleotides

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Cover of 'Therapeutic Oligonucleotides'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Therapeutic oligonucleotides.
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    Chapter 2 Therapeutic Oligonucleotides
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    Chapter 3 2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNACOC)
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    Chapter 4 A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery.
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    Chapter 5 Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides.
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    Chapter 6 A nanoparticle for tumor targeted delivery of oligomers.
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    Chapter 7 Light-Directed Delivery of Nucleic Acids
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    Chapter 8 Antibody Targeted siRNA Delivery.
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    Chapter 9 Aptamer-drug conjugation for targeted tumor cell therapy.
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    Chapter 10 Five-Step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
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    Chapter 11 Diverse Small Non-coding RNAs in RNA Interference Pathways.
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    Chapter 12 Quantification of siRNAs In Vitro and In Vivo.
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    Chapter 13 Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.
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    Chapter 14 siRNA Knockdown of Gene Expression in Endothelial Cells.
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    Chapter 15 Using RNA Interference in Schistosoma mansoni
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    Chapter 16 Performing the Labeled microRNA Pull-Down (LAMP) Assay System: An Experimental Approach for High-Throughput Identification of microRNA-Target mRNAs.
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    Chapter 17 Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8
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    Chapter 18 Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-Like Receptor 9
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    Chapter 19 Surface Plasmon Resonance Investigation of RNA Aptamer–RNA Ligand Interactions
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    Chapter 20 Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein
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    Chapter 21 Inhibition of Human Papillomavirus Expression Using DNAzymes
Attention for Chapter 13: Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.
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Chapter title
Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR.
Chapter number 13
Book title
Therapeutic Oligonucleotides
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-188-8_13
Pubmed ID
Book ISBNs
978-1-61779-187-1, 978-1-61779-188-8
Authors

Angie Cheng, Susan Magdaleno, Alexander V. Vlassov

Abstract

RNA interference (RNAi) is a mechanism by which the introduction of small interfering RNAs (siRNAs) into cultured cells causes degradation of the complementary mRNA. Applications of RNAi include gene function analysis, pathway analysis, and target validation. While RNAi experiments have become common practice in research labs, multiple factors can influence the extent of siRNA-induced knockdown (and thus biological outcome). A properly designed and selected siRNA sequence, siRNA modification format, choice of transfection reagent/technique, optimized protocols of siRNA in vitro delivery, and an appropriate and optimized readout are all critical for ensuring a successful experiment. In this chapter, we describe a typical in vitro siRNA experiment with optimization of transfection conditions and analysis of siRNA potency, i.e., mRNA knockdown with quantitative real-time PCR.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 4%
Colombia 1 4%
Switzerland 1 4%
Unknown 25 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 36%
Student > Master 5 18%
Researcher 4 14%
Student > Bachelor 3 11%
Other 2 7%
Other 4 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 54%
Biochemistry, Genetics and Molecular Biology 4 14%
Medicine and Dentistry 3 11%
Chemistry 2 7%
Social Sciences 1 4%
Other 2 7%
Unknown 1 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2011.
All research outputs
#3,108,413
of 4,506,407 outputs
Outputs from Methods in molecular biology
#1,458
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Outputs of similar age
#36,453
of 54,728 outputs
Outputs of similar age from Methods in molecular biology
#8
of 9 outputs
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