Title |
Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting
|
---|---|
Published in |
BMC Infectious Diseases, October 2014
|
DOI | 10.1186/s12879-014-0558-4 |
Pubmed ID | |
Authors |
Maria Cristina Carvalho Espírito-Santo, Mónica Viviana Alvarado-Mora, Emmanuel Dias-Neto, Lívia Souza Botelho-Lima, João Paulo Moreira, Maria Amorim, Pedro Luiz Silva Pinto, Ashley R Heath, Vera Lúcia Pagliusi Castilho, Elenice Messias do Nascimento Gonçalves, Expedito José de Albuquerque Luna, Flair José Carrilho, João Renato Rebello Pinho, Ronaldo Cesar Borges Gryschek |
Abstract |
Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, schistosomiasis has been reported in 19 states, showing areas of high and medium endemicity and a wide range of areas of low endemicity (ALE). Barra Mansa in Rio de Janeiro state has an estimated prevalence of 1%. ALE represent a new challenge for the helminth control because about 75% of infected individuals are asymptomatic and infections occur with a low parasite load (<100 eggs per gram of feces), causing a decrease in sensitivity of stool parasitological techniques, which are a reference for the laboratory diagnosis of this helminth. The objective of this study was to evaluate the performance of a TaqMan quantitative polymerase chain reaction (qPCR) technique in serum and feces DNA samples using the techniques of Kato-Katz (KK), Hoffman, Pons and Janer (HH) as references, during an epidemiological survey using fecal samples and sera from randomized residents from an ALE. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Burkina Faso | 1 | 2% |
Brazil | 1 | 2% |
Unknown | 54 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 11 | 20% |
Student > Bachelor | 9 | 16% |
Student > Ph. D. Student | 6 | 11% |
Researcher | 4 | 7% |
Professor | 3 | 5% |
Other | 8 | 14% |
Unknown | 15 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 13 | 23% |
Biochemistry, Genetics and Molecular Biology | 12 | 21% |
Agricultural and Biological Sciences | 6 | 11% |
Nursing and Health Professions | 4 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 5 | 9% |
Unknown | 15 | 27% |