Title |
In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study
|
---|---|
Published in |
EBioMedicine, September 2014
|
DOI | 10.1016/j.ebiom.2014.09.001 |
Pubmed ID | |
Authors |
Michael Olausson, Vijay Kumar Kuna, Galyna Travnikova, Henrik Bäckdahl, Pradeep B. Patil, Robert Saalman, Helena Borg, Anders Jeppsson, Suchitra Sumitran-Holgersson |
Abstract |
Vascular diseases are increasing health problems affecting > 25 million individuals in westernized societies. Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Here we demonstrate a novel approach to generate transplantable vascular grafts using decellularized allogeneic vascular scaffolds, repopulated with peripheral whole blood (PWB) in vitro in a bioreactor. Circulating, VEGFR-2 +/CD45 + and a smaller fraction of VEGFR-2 +/CD14 + cells contributed to repopulation of the graft. SEM micrographs showed flat cells on the luminal surface of the grafts consistent with endothelial cells. For clinical validation, two autologous PWB tissue-engineered vein conduits were prepared and successfully used for by-pass procedures in two pediatric patients. These results provide a proof of principle for the generation of transplantable vascular grafts using a simple autologous blood sample, making it clinically feasible globally. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Italy | 5 | 29% |
United States | 2 | 12% |
Sweden | 2 | 12% |
United Kingdom | 2 | 12% |
India | 1 | 6% |
Unknown | 5 | 29% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 13 | 76% |
Practitioners (doctors, other healthcare professionals) | 2 | 12% |
Scientists | 1 | 6% |
Science communicators (journalists, bloggers, editors) | 1 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 1% |
Czechia | 1 | 1% |
United Kingdom | 1 | 1% |
Russia | 1 | 1% |
Spain | 1 | 1% |
United States | 1 | 1% |
Unknown | 79 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 16 | 19% |
Student > Ph. D. Student | 14 | 16% |
Student > Master | 14 | 16% |
Student > Bachelor | 9 | 11% |
Professor > Associate Professor | 4 | 5% |
Other | 11 | 13% |
Unknown | 17 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 21% |
Medicine and Dentistry | 13 | 15% |
Engineering | 11 | 13% |
Biochemistry, Genetics and Molecular Biology | 7 | 8% |
Materials Science | 4 | 5% |
Other | 6 | 7% |
Unknown | 26 | 31% |