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Duchenne Muscular Dystrophy

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Cover of 'Duchenne Muscular Dystrophy'

Table of Contents

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    Book Overview
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    Chapter 1 An Overview of Recent Therapeutics Advances for Duchenne Muscular Dystrophy
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    Chapter 2 Clinical Manifestations and Overall Management Strategies for Duchenne Muscular Dystrophy
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    Chapter 3 Cardiac Involvement in Duchenne Muscular Dystrophy and Related Dystrophinopathies
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    Chapter 4 Characterization of the Inflammatory Response in Dystrophic Muscle Using Flow Cytometry
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    Chapter 5 Imaging Analysis of the Neuromuscular Junction in Dystrophic Muscle
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    Chapter 6 System Biology Approach: Gene Network Analysis for Muscular Dystrophy
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    Chapter 7 Proteomic Profiling of the Dystrophin-Deficient Brain
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    Chapter 8 Probing the Pathogenesis of Duchenne Muscular Dystrophy Using Mouse Models
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    Chapter 9 Exon Skipping Therapy Using Phosphorodiamidate Morpholino Oligomers in the mdx52 Mouse Model of Duchenne Muscular Dystrophy
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    Chapter 10 Designing Effective Antisense Oligonucleotides for Exon Skipping
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    Chapter 11 Identification of Splicing Factors Involved in DMD Exon Skipping Events Using an In Vitro RNA Binding Assay
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    Chapter 12 The Use of Antisense Oligonucleotides for the Treatment of Duchenne Muscular Dystrophy
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    Chapter 13 PMO Delivery System Using Bubble Liposomes and Ultrasound Exposure for Duchenne Muscular Dystrophy Treatment
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    Chapter 14 Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD)
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    Chapter 15 Test of Antifibrotic Drugs in a Cellular Model of Fibrosis Based on Muscle-Derived Fibroblasts from Duchenne Muscular Dystrophy Patients
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    Chapter 16 Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy
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    Chapter 17 Advanced Methods to Study the Cross Talk Between Fibro-Adipogenic Progenitors and Muscle Stem Cells
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    Chapter 18 AAV6 Vector Production and Purification for Muscle Gene Therapy
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    Chapter 19 From gRNA Identification to the Restoration of Dystrophin Expression: A Dystrophin Gene Correction Strategy for Duchenne Muscular Dystrophy Mutations Using the CRISPR-Induced Deletion Method
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    Chapter 20 Erratum to: Functional Analysis of Membrane Proteins Produced by Cell-Free Translation
Attention for Chapter 14: Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD)
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Chapter title
Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD)
Chapter number 14
Book title
Duchenne Muscular Dystrophy
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7374-3_14
Pubmed ID
Book ISBNs
978-1-4939-7373-6, 978-1-4939-7374-3
Authors

Niraj Kumar Srivastava

Abstract

Proton nuclear magnetic resonance spectroscopy ((1)H NMR) is a useful tool for the analysis of lipid components in biofluids such as serum/plasma. Such tool is applied for the analysis of lipid components in serum/plasma of patients with Duchenne muscular dystrophy (DMD). The practical approach of sampling, storage, lipid extraction procedure, sample preparation before performing the (1)H NMR spectroscopy experiments is presented. All the experimental parameters of NMR spectroscopy are also described. Details of the assignments of lipid components (qualitative analysis) and quantification of particular lipid components (quantitative analysis) are explained.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 14%
Researcher 1 14%
Other 1 14%
Unknown 4 57%
Readers by discipline Count As %
Unspecified 1 14%
Nursing and Health Professions 1 14%
Chemistry 1 14%
Unknown 4 57%