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SARS- and Other Coronaviruses

Overview of attention for book
Cover of 'SARS- and Other Coronaviruses'

Table of Contents

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    Book Overview
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    Chapter 1 A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
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    Chapter 2 Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions
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    Chapter 3 Detection and Sequence Characterization of the 3′-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m
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    Chapter 4 RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
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    Chapter 5 Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains
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    Chapter 6 Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
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    Chapter 7 Detection of New Viruses by VIDISCA
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    Chapter 8 Titration of human coronaviruses, HcoV-229E and HCoV-OC43, by an indirect immunoperoxidase assay.
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    Chapter 9 The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
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    Chapter 10 Isolation and propagation of coronaviruses in embryonated eggs.
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    Chapter 11 Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen
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    Chapter 12 Purification and Electron Cryomicroscopy of Coronavirus Particles
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    Chapter 13 Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization
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    Chapter 14 Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus
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    Chapter 15 Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
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    Chapter 16 Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins
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    Chapter 17 Manipulation of the Coronavirus Genome Using Targeted RNA Recombination with Interspecies Chimeric Coronaviruses
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    Chapter 18 Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs
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    Chapter 19 Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses
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    Chapter 20 Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
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    Chapter 21 Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome
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    Chapter 22 Identification of sugar residues involved in the binding of TGEV to porcine brush border membranes.
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    Chapter 23 Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins
Attention for Chapter 8: Titration of human coronaviruses, HcoV-229E and HCoV-OC43, by an indirect immunoperoxidase assay.
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About this Attention Score

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  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

1 tweeter
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Chapter title
Titration of human coronaviruses, HcoV-229E and HCoV-OC43, by an indirect immunoperoxidase assay.
Chapter number 8
Book title
SARS- and Other Coronaviruses
Published in
Methods in molecular biology, December 2008
DOI 10.1007/978-1-59745-181-9_8
Pubmed ID
Book ISBNs
978-1-58829-867-6, 978-1-59745-181-9

Francine Lambert, Hélène Jacomy, Gabriel Marceau, Pierre J Talbot*


Calculation of infectious viral titers represents a basic and essential experimental approach for virologists. Classical plaque assays cannot be used for viruses that do not cause significant cytopathic effects, which is the case for strains 229E and OC43 of human coronavirus (HCoV). An alternative indirect immunoperoxidase assay (IPA) is herein described for the detection and titration of these viruses. Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96-well plate. After viral growth, viral detection by IPA yields the infectious virus titer, expressed as "Tissue Culture Infectious Dose" (TCID50). This represents the dilution of a virus-containing sample at which half of a series of laboratory wells contain replicating virus. This technique is a reliable method for the titration of HCoV in biological samples (cells, tissues, or fluids).

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 33%
Professor > Associate Professor 3 25%
Student > Ph. D. Student 1 8%
Professor 1 8%
Student > Master 1 8%
Other 2 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 25%
Medicine and Dentistry 2 17%
Unspecified 2 17%
Nursing and Health Professions 1 8%
Business, Management and Accounting 1 8%
Other 3 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2020.
All research outputs
of 14,737,502 outputs
Outputs from Methods in molecular biology
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Outputs of similar age
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Outputs of similar age from Methods in molecular biology
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Altmetric has tracked 14,737,502 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 8,777 research outputs from this source. They receive a mean Attention Score of 2.5. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 217,821 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.