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Comparing the physiochemical parameters of three celluloses reveals new insights into substrate suitability for fungal enzyme production

Overview of attention for article published in Fungal Biology and Biotechnology, November 2017
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Title
Comparing the physiochemical parameters of three celluloses reveals new insights into substrate suitability for fungal enzyme production
Published in
Fungal Biology and Biotechnology, November 2017
DOI 10.1186/s40694-017-0039-9
Pubmed ID
Authors

Lara Hassan, Manfred J. Reppke, Nils Thieme, Steffen A. Schweizer, Carsten W. Mueller, J. Philipp Benz

Abstract

The industrial applications of cellulases are mostly limited by the costs associated with their production. Optimized production pathways are therefore desirable. Based on their enzyme inducing capacity, celluloses are commonly used in fermentation media. However, the influence of their physiochemical characteristics on the production process is not well understood. In this study, we examined how physical, structural and chemical properties of celluloses influence cellulase and hemicellulase production in an industrially-optimized and a non-engineered filamentous fungus: Trichoderma reesei RUT-C30 and Neurospora crassa. The performance was evaluated by quantifying gene induction, protein secretion and enzymatic activities. Among the three investigated substrates, the powdered cellulose was found to be the most impure, and the residual hemicellulosic content was efficiently perceived by the fungi. It was furthermore found to be the least crystalline substrate and consequently was the most readily digested cellulose in vitro. In vivo however, only RUT-C30 was able to take full advantage of these factors. When comparing carbon catabolite repressed and de-repressed strains of T. reesei and N. crassa, we found that cre1/cre-1 is at least partially responsible for this observation, but that the different wiring of the molecular signaling networks is also relevant. Our findings indicate that crystallinity and hemicellulose content are major determinants of performance. Moreover, the genetic background between WT and modified strains greatly affects the ability to utilize the cellulosic substrate. By highlighting key factors to consider when choosing the optimal cellulosic product for enzyme production, this study has relevance for the optimization of a critical step in the biotechnological (hemi-) cellulase production process.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 33%
Researcher 4 27%
Student > Bachelor 2 13%
Professor 1 7%
Student > Master 1 7%
Other 1 7%
Unknown 1 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 47%
Biochemistry, Genetics and Molecular Biology 3 20%
Arts and Humanities 1 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Unspecified 1 7%
Other 2 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2017.
All research outputs
#9,669,649
of 12,091,568 outputs
Outputs from Fungal Biology and Biotechnology
#48
of 54 outputs
Outputs of similar age
#206,535
of 284,599 outputs
Outputs of similar age from Fungal Biology and Biotechnology
#7
of 7 outputs
Altmetric has tracked 12,091,568 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 54 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 284,599 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one.