Title |
Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland.
|
---|---|
Published in |
Annals of parasitology, January 2011
|
Pubmed ID | |
Authors |
Łukasz Adaszek, Marta Górna, Michał Krzysiak, Michał Adaszek, Marcin Garbal, Stanisław Winiarczyk |
Abstract |
The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products demonstrated a 95.6-97.5% similarity with the sequence of the fragment of 18S RNA Babesia equi, gene number DQ287951 in the GenBank. The treatment utilizing the subcutaneous application of the imidocarb resulted in gradual recovery of the diseased animals. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 21% |
Lecturer > Senior Lecturer | 2 | 8% |
Researcher | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Professor > Associate Professor | 2 | 8% |
Other | 7 | 29% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 11 | 46% |
Agricultural and Biological Sciences | 3 | 13% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
Environmental Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 3 | 13% |
Unknown | 3 | 13% |