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Argonaute Proteins

Overview of attention for book
Cover of 'Argonaute Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing
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    Chapter 2 Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins Using SYBR Green-Based qRT-PCR
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    Chapter 3 Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples
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    Chapter 4 Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant
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    Chapter 5 MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans
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    Chapter 6 cCLIP-Seq: Retrieval of Chimeric Reads from HITS-CLIP (CLIP-Seq) Libraries
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    Chapter 7 Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro
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    Chapter 8 Nucleic Acid-Binding Assay of Argonaute Protein Using Fluorescence Polarization
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    Chapter 9 Reconstitution of RNA Interference Machinery
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    Chapter 10 Single-Molecule Analysis for RISC Assembly and Target Cleavage
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    Chapter 11 Profiling Open Chromatin Structure in the Ovarian Somatic Cells Using ATAC-seq
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    Chapter 12 Assessing miR-451 Activity and Its Role in Erythropoiesis
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    Chapter 13 Functional Analysis of MicroRNAs in Neurogenesis During Mouse Cortical Development
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    Chapter 14 Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing
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    Chapter 15 Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination
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    Chapter 16 Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A Vade Mecum for MINTmap
Attention for Chapter 15: Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination
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Chapter title
Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination
Chapter number 15
Book title
Argonaute Proteins
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7339-2_15
Pubmed ID
29030852
Book ISBNs
978-1-4939-7338-5, 978-1-4939-7339-2
Authors

Sanjay Ghosh, Ji-Long Liu

Abstract

Tagging of genes at the endogenous loci is a powerful strategy for the analysis of protein function. We have developed a homologous recombination-based approach for inserting epitope tag into Drosophila AGO1 locus by employing the CRISPR/Cas9 technology. The methodology involves co-expression of sgRNA (containing 20-nucleotide AGO1 targeting sequence) and Cas9 protein, together with a donor template that has HA-AGO1 cassette flanked by sequences homologous to the AGO1 locus. The integration is efficient and readily monitored by immunostaining of the transgenic cell line. This method facilitates rapid generation of stable cell lines and allows insertion of any tag sequence into endogenous loci, thus accelerating characterization of the tagged proteins.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Student > Bachelor 3 30%
Student > Ph. D. Student 1 10%
Unknown 3 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 40%
Biochemistry, Genetics and Molecular Biology 3 30%
Unknown 3 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2017.
All research outputs
#20,450,513
of 23,006,268 outputs
Outputs from Methods in molecular biology
#9,941
of 13,160 outputs
Outputs of similar age
#378,088
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#1,193
of 1,498 outputs
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So far Altmetric has tracked 13,160 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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