↓ Skip to main content
What is this page? Embed badge

Argonaute Proteins

Overview of attention for book
Cover of 'Argonaute Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing
  3. Altmetric Badge
    Chapter 2 Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins Using SYBR Green-Based qRT-PCR
  4. Altmetric Badge
    Chapter 3 Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples
  5. Altmetric Badge
    Chapter 4 Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant
  6. Altmetric Badge
    Chapter 5 MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans
  7. Altmetric Badge
    Chapter 6 cCLIP-Seq: Retrieval of Chimeric Reads from HITS-CLIP (CLIP-Seq) Libraries
  8. Altmetric Badge
    Chapter 7 Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro
  9. Altmetric Badge
    Chapter 8 Nucleic Acid-Binding Assay of Argonaute Protein Using Fluorescence Polarization
  10. Altmetric Badge
    Chapter 9 Reconstitution of RNA Interference Machinery
  11. Altmetric Badge
    Chapter 10 Single-Molecule Analysis for RISC Assembly and Target Cleavage
  12. Altmetric Badge
    Chapter 11 Profiling Open Chromatin Structure in the Ovarian Somatic Cells Using ATAC-seq
  13. Altmetric Badge
    Chapter 12 Assessing miR-451 Activity and Its Role in Erythropoiesis
  14. Altmetric Badge
    Chapter 13 Functional Analysis of MicroRNAs in Neurogenesis During Mouse Cortical Development
  15. Altmetric Badge
    Chapter 14 Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing
  16. Altmetric Badge
    Chapter 15 Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination
  17. Altmetric Badge
    Chapter 16 Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A Vade Mecum for MINTmap
Attention for Chapter 14: Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing
Altmetric Badge

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
4 Mendeley
Summary Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing
Chapter number 14
Book title
Argonaute Proteins
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7339-2_14
Pubmed ID
29030851
Book ISBNs
978-1-4939-7338-5, 978-1-4939-7339-2
Authors

Cai Zhang, Joonbae Seo, Takahisa Nakamura

Abstract

In mammals, there are four Argonaute (Ago) family proteins that play crucial roles in RNA silencing, a process wherein microRNA (miRNA) mediates inhibition of target mRNA translation. Among the Ago proteins, Argonaute2 (Ago2) uniquely possesses an endoribonuclease (slicer) activity that is critical for the biogenesis of specific miRNAs and mRNA cleavage. This Ago2 slicer activity is required for postnatal development. Despite its important roles, there are still gaps in our understanding of the mechanistic basis of Ago2's unique functions in vivo due to a limited availability of experimental tools. In order to investigate Ago2's functions, we generated a new cellular model of Ago2-deficiency in 3T3 mouse embryonic fibroblasts (MEFs). This cell line can be used for investigating general Ago2 functions, but also for further understanding of Ago2's unique characteristics including the slicer activity, specific amino acid residues, and domains in Ago2 by reconstitution of Ago2 mutants. Here, we describe the methods for establishing Ago2-deficient MEFs and for reconstituting the MEFs with an Ago2 mutant lacking its slicer activity by means of a retrovirus-mediated gene transfer.

View on publisher site
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Researcher 1 25%
Other 1 25%
Unknown 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 25%
Agricultural and Biological Sciences 1 25%
Unknown 2 50%

This page is provided by Altmetric.