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Argonaute Proteins

Overview of attention for book
Cover of 'Argonaute Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing
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    Chapter 2 Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins Using SYBR Green-Based qRT-PCR
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    Chapter 3 Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples
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    Chapter 4 Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant
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    Chapter 5 MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans
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    Chapter 6 cCLIP-Seq: Retrieval of Chimeric Reads from HITS-CLIP (CLIP-Seq) Libraries
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    Chapter 7 Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro
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    Chapter 8 Nucleic Acid-Binding Assay of Argonaute Protein Using Fluorescence Polarization
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    Chapter 9 Reconstitution of RNA Interference Machinery
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    Chapter 10 Single-Molecule Analysis for RISC Assembly and Target Cleavage
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    Chapter 11 Profiling Open Chromatin Structure in the Ovarian Somatic Cells Using ATAC-seq
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    Chapter 12 Assessing miR-451 Activity and Its Role in Erythropoiesis
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    Chapter 13 Functional Analysis of MicroRNAs in Neurogenesis During Mouse Cortical Development
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    Chapter 14 Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing
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    Chapter 15 Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination
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    Chapter 16 Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A Vade Mecum for MINTmap
Attention for Chapter 12: Assessing miR-451 Activity and Its Role in Erythropoiesis
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Chapter title
Assessing miR-451 Activity and Its Role in Erythropoiesis
Chapter number 12
Book title
Argonaute Proteins
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7339-2_12
Pubmed ID
Book ISBNs
978-1-4939-7338-5, 978-1-4939-7339-2
Authors

Dmitry A. Kretov, Andrew M. Shafik, Daniel Cifuentes, Kretov, Dmitry A., Shafik, Andrew M., Cifuentes, Daniel

Abstract

The ability to microinject small RNAs and mRNAs into zebrafish embryos, of different genetic backgrounds, allows for the precise dissection of microRNA processing pathways at the molecular level, while simultaneously provides insight into their physiologic role. Here, we apply such an approach to determine the impact of Argonaute 2 in the processing of miR-451, a vertebrate-specific microRNA required for terminal erythrocyte differentiation. This was achieved using fluorescent microRNA reporter sensor assays and phenotype rescue experiments.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Student > Doctoral Student 2 25%
Student > Ph. D. Student 1 13%
Student > Postgraduate 1 13%
Unspecified 1 13%
Other 0 0%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 50%
Unspecified 1 13%
Computer Science 1 13%
Agricultural and Biological Sciences 1 13%
Unknown 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2018.
All research outputs
#15,482,347
of 23,007,053 outputs
Outputs from Methods in molecular biology
#5,388
of 13,157 outputs
Outputs of similar age
#269,706
of 442,275 outputs
Outputs of similar age from Methods in molecular biology
#596
of 1,498 outputs
Altmetric has tracked 23,007,053 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,275 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.