| Chapter title |
Reconstitution of RNA Interference Machinery
|
|---|---|
| Chapter number | 9 |
| Book title |
Argonaute Proteins
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7339-2_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7338-5, 978-1-4939-7339-2
|
| Authors |
Shintaro Iwasaki, Yukihide Tomari, Iwasaki, Shintaro, Tomari, Yukihide |
| Abstract |
Small RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs), silence protein expression from target mRNAs bearing their complementary sequences, via the formation of the effector complex called RNA-induced silencing complex (RISC). Although the mechanism of RISC assembly has been studied for nearly two decades, the detailed mechanism has still remained unclear in part due to the lack of a pure reconstitution system. Recently, we identified all the core proteins necessary for RISC assembly in flies and successfully recapitulated the assembly of catalytically active RISC with eight recombinant proteins. The reconstitution system provides a versatile framework for detailed studies of RISC assembly, including single molecule analysis as described in another chapter in this issue. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 22% |
| Researcher | 2 | 22% |
| Student > Bachelor | 1 | 11% |
| Unspecified | 1 | 11% |
| Student > Master | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 33% |
| Biochemistry, Genetics and Molecular Biology | 3 | 33% |
| Arts and Humanities | 1 | 11% |
| Unspecified | 1 | 11% |
| Unknown | 1 | 11% |