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Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2

Overview of attention for article published in BMC Veterinary Research, November 2017
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Title
Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2
Published in
BMC Veterinary Research, November 2017
DOI 10.1186/s12917-017-1232-z
Pubmed ID
Authors

Yunyun Geng, Jianchang Wang, Libing Liu, Yan Lu, Ke Tan, Yan-Zhong Chang

Abstract

Canine parvovirus 2, a linear single-stranded DNA virus belonging to the genus Parvovirus within the family Parvoviridae, is a highly contagious pathogen of domestic dogs and several wild canidae species. Early detection of canine parvovirus (CPV-2) is crucial to initiating appropriate outbreak control strategies. Recombinase polymerase amplification (RPA), a novel isothermal gene amplification technique, has been developed for the molecular detection of diverse pathogens. In this study, a real-time RPA assay was developed for the detection of CPV-2 using primers and an exo probe targeting the CPV-2 nucleocapsid protein gene. The real-time RPA assay was performed successfully at 38 °C, and the results were obtained within 4-12 min for 10(5)-10(1) molecules of template DNA. The assay only detected CPV-2, and did not show cross-detection of other viral pathogens, demonstrating a high level of specificity. The analytical sensitivity of the real-time RPA was 10(1) copies/reaction of a standard DNA template, which was 10 times more sensitive than the common RPA method. The clinical sensitivity of the real-time RPA assay matched 100% (n = 91) to the real-time PCR results. The real-time RPA assay is a simple, rapid, reliable and affordable method that can potentially be applied for the detection of CPV-2 in the research laboratory and point-of-care diagnosis.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 23%
Student > Bachelor 1 8%
Other 1 8%
Student > Ph. D. Student 1 8%
Researcher 1 8%
Other 0 0%
Unknown 6 46%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 3 23%
Medicine and Dentistry 2 15%
Biochemistry, Genetics and Molecular Biology 1 8%
Unknown 7 54%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2017.
All research outputs
#9,686,742
of 12,114,099 outputs
Outputs from BMC Veterinary Research
#1,089
of 1,666 outputs
Outputs of similar age
#206,934
of 285,227 outputs
Outputs of similar age from BMC Veterinary Research
#99
of 139 outputs
Altmetric has tracked 12,114,099 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,666 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 285,227 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 139 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.