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Bacteriophages

Overview of attention for book
Cover of 'Bacteriophages'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Basic Phage Mathematics
  3. Altmetric Badge
    Chapter 2 Analysis of Host-Takeover During SPO1 Infection of Bacillus subtilis
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    Chapter 3 Practical Advice on the One-Step Growth Curve
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    Chapter 4 Iron Chloride Flocculation of Bacteriophages from Seawater
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    Chapter 5 Purification of Bacteriophages Using Anion-Exchange Chromatography
  7. Altmetric Badge
    Chapter 6 Encapsulation Strategies of Bacteriophage (Felix O1) for Oral Therapeutic Application
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    Chapter 7 Encapsulation of Listeria Phage A511 by Alginate to Improve Its Thermal Stability
  9. Altmetric Badge
    Chapter 8 Application of a Virucidal Agent to Avoid Overestimation of Phage Kill During Phage Decontamination Assays on Ready-to-Eat Meats
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    Chapter 9 Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes
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    Chapter 10 Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology
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    Chapter 11 Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing
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    Chapter 12 Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques Using SISPA
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    Chapter 13 Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque
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    Chapter 14 Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq
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    Chapter 15 Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis
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    Chapter 16 Annotation of Bacteriophage Genome Sequences Using DNA Master: An Overview
  18. Altmetric Badge
    Chapter 17 Phage Genome Annotation Using the RAST Pipeline
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    Chapter 18 Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams
  20. Altmetric Badge
    Chapter 19 Transposable Bacteriophages as Genetic Tools
  21. Altmetric Badge
    Chapter 20 Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes
  22. Altmetric Badge
    Chapter 21 Use of RP4::Mini-Mu for Gene Transfer
  23. Altmetric Badge
    Chapter 22 Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu
Attention for Chapter 10: Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology
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Chapter title
Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology
Chapter number 10
Book title
Bacteriophages
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7343-9_10
Pubmed ID
Book ISBNs
978-1-4939-7341-5, 978-1-4939-7343-9
Authors

Philip Kelleher, James Murphy, Jennifer Mahony, Douwe van Sinderen

Abstract

Whole phage genomes can be sequenced readily using one or a combination of next generation sequencing (NGS) technologies. One of the most recently developed NGS platforms, the so-called Single-Molecule Real-Time (SMRT) sequencing approach provided by the PacBio RS platform, is particularly useful in providing complete (i.e., un-gapped) genome sequences, but differs from other technologies in that the platform also allows for downstream analysis to identify nucleotides that have been modified by DNA methylation. Here, we describe the methodological approach for the detection of genomic methylation motifs by means of SMRT sequencing.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 29%
Student > Ph. D. Student 3 21%
Student > Bachelor 2 14%
Lecturer 1 7%
Unknown 4 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 50%
Biochemistry, Genetics and Molecular Biology 3 21%
Unknown 4 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 June 2018.
All research outputs
#17,919,786
of 23,007,887 outputs
Outputs from Methods in molecular biology
#7,286
of 13,157 outputs
Outputs of similar age
#310,312
of 442,278 outputs
Outputs of similar age from Methods in molecular biology
#868
of 1,498 outputs
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So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.