Title |
Chromatin accessibility: a window into the genome
|
---|---|
Published in |
Epigenetics & Chromatin, November 2014
|
DOI | 10.1186/1756-8935-7-33 |
Pubmed ID | |
Authors |
Maria Tsompana, Michael J Buck |
Abstract |
Transcriptional activation throughout the eukaryotic lineage has been tightly linked with disruption of nucleosome organization at promoters, enhancers, silencers, insulators and locus control regions due to transcription factor binding. Regulatory DNA thus coincides with open or accessible genomic sites of remodeled chromatin. Current chromatin accessibility assays are used to separate the genome by enzymatic or chemical means and isolate either the accessible or protected locations. The isolated DNA is then quantified using a next-generation sequencing platform. Wide application of these assays has recently focused on the identification of the instrumental epigenetic changes responsible for differential gene expression, cell proliferation, functional diversification and disease development. Here we discuss the limitations and advantages of current genome-wide chromatin accessibility assays with especial attention on experimental precautions and sequence data analysis. We conclude with our perspective on future improvements necessary for moving the field of chromatin profiling forward. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 4 | 31% |
United States | 1 | 8% |
France | 1 | 8% |
Germany | 1 | 8% |
Canada | 1 | 8% |
Unknown | 5 | 38% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 7 | 54% |
Scientists | 4 | 31% |
Science communicators (journalists, bloggers, editors) | 2 | 15% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 16 | 1% |
France | 3 | <1% |
United Kingdom | 3 | <1% |
Germany | 2 | <1% |
Korea, Republic of | 2 | <1% |
Denmark | 2 | <1% |
Japan | 2 | <1% |
Israel | 1 | <1% |
Canada | 1 | <1% |
Other | 4 | <1% |
Unknown | 1154 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 317 | 27% |
Researcher | 205 | 17% |
Student > Master | 162 | 14% |
Student > Bachelor | 119 | 10% |
Student > Doctoral Student | 66 | 6% |
Other | 116 | 10% |
Unknown | 205 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 382 | 32% |
Agricultural and Biological Sciences | 373 | 31% |
Medicine and Dentistry | 39 | 3% |
Computer Science | 33 | 3% |
Neuroscience | 30 | 3% |
Other | 100 | 8% |
Unknown | 233 | 20% |