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Altered expression of Arabidopsis genes in response to a multifunctional geminivirus pathogenicity protein

Overview of attention for article published in BMC Plant Biology, November 2014
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Title
Altered expression of Arabidopsis genes in response to a multifunctional geminivirus pathogenicity protein
Published in
BMC Plant Biology, November 2014
DOI 10.1186/s12870-014-0302-7
Pubmed ID
Authors

Lu Liu, Ho Yong Chung, Gabriela Lacatus, Surendranath Baliji, Jianhua Ruan, Garry Sunter

Abstract

BackgroundGeminivirus AC2 is a multifunctional protein that acts as a pathogenicity factor. Transcriptional regulation by AC2 appears to be mediated through interaction with a plant specific DNA binding protein, PEAPOD2 (PPD2), that specifically binds to sequences known to mediate activation of the CP promoter of Cabbage leaf curl virus (CaLCuV) and Tomato golden mosaic virus (TGMV). Suppression of both basal and innate immune responses by AC2 in plants is mediated through inactivation of SnRK1.2, an Arabidopsis SNF1 related protein kinase, and adenosine kinase (ADK). An indirect promoter targeting strategy, via AC2-host dsDNA binding protein interactions, and inactivation of SnRK1.2-mediated defense responses could provide the opportunity for geminiviruses to alter host gene expression and in turn, reprogram the host to support virus infection. The goal of this study was to identify changes in the transcriptome of Arabidopsis induced by the transcription activation function of AC2 and the inactivation of SnRK1.2.ResultsUsing full-length and truncated AC2 proteins, microarray analyses identified 834 genes differentially expressed in response to the transcriptional regulatory function of the AC2 protein at one and two days post treatment. We also identified 499 genes differentially expressed in response to inactivation of SnRK1.2 by the AC2 protein at one and two days post treatment. Network analysis of these two sets of differentially regulated genes identified several networks consisting of between four and eight highly connected genes. Quantitative real-time PCR analysis validated the microarray expression results for 10 out of 11 genes tested.ConclusionsIt is becoming increasingly apparent that geminiviruses manipulate the host in several ways to facilitate an environment conducive to infection, predominantly through the use of multifunctional proteins. Our approach of identifying networks of highly connected genes that are potentially co-regulated by geminiviruses during infection will allow us to identify novel pathways of co-regulated genes that are stimulated in response to pathogen infection in general, and virus infection in particular.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 2%
South Africa 1 2%
Unknown 50 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 27%
Student > Master 10 19%
Researcher 4 8%
Student > Doctoral Student 3 6%
Other 2 4%
Other 7 13%
Unknown 12 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 46%
Biochemistry, Genetics and Molecular Biology 11 21%
Medicine and Dentistry 2 4%
Immunology and Microbiology 1 2%
Chemical Engineering 1 2%
Other 0 0%
Unknown 13 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2014.
All research outputs
#11,030,038
of 12,410,450 outputs
Outputs from BMC Plant Biology
#1,230
of 1,566 outputs
Outputs of similar age
#224,514
of 276,408 outputs
Outputs of similar age from BMC Plant Biology
#153
of 186 outputs
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