↓ Skip to main content

RNA Detection

Overview of attention for book
Cover of 'RNA Detection'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Secret Life of RNA: Lessons from Emerging Methodologies
  3. Altmetric Badge
    Chapter 2 Quantification of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
  4. Altmetric Badge
    Chapter 3 Identifying the m6A Methylome by Affinity Purification and Sequencing
  5. Altmetric Badge
    Chapter 4 PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
  6. Altmetric Badge
    Chapter 5 Axon-TRAP-RiboTag: Affinity Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
  7. Altmetric Badge
    Chapter 6 LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
  8. Altmetric Badge
    Chapter 7 Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
  9. Altmetric Badge
    Chapter 8 Single mRNA Molecule Detection in Drosophila
  10. Altmetric Badge
    Chapter 9 Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
  11. Altmetric Badge
    Chapter 10 Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
  12. Altmetric Badge
    Chapter 11 Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
  13. Altmetric Badge
    Chapter 12 Hybridization Chain Reaction for Direct mRNA Detection Without Nucleic Acid Purification
  14. Altmetric Badge
    Chapter 13 In Situ Detection of MicroRNA Expression with RNAscope Probes
  15. Altmetric Badge
    Chapter 14 Padlock Probes to Detect Single Nucleotide Polymorphisms
  16. Altmetric Badge
    Chapter 15 Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
  17. Altmetric Badge
    Chapter 16 Optimizing Molecular Beacons for Intracellular Analysis of RNA
  18. Altmetric Badge
    Chapter 17 Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
  19. Altmetric Badge
    Chapter 18 In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes
  20. Altmetric Badge
    Chapter 19 Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers
  21. Altmetric Badge
    Chapter 20 Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe
  22. Altmetric Badge
    Chapter 21 RNA Live Imaging in the Model Microorganism Ustilago maydis
  23. Altmetric Badge
    Chapter 22 Real-Time Fluorescence Imaging of Single-Molecule Endogenous Noncoding RNA in Living Cells
  24. Altmetric Badge
    Chapter 23 Live Imaging of mRNA Synthesis in Drosophila
  25. Altmetric Badge
    Chapter 24 Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
  26. Altmetric Badge
    Chapter 25 Detection of the First Round of Translation: The TRICK Assay
  27. Altmetric Badge
    Chapter 26 Imaging Translation Dynamics of Single mRNA Molecules in Live Cells
  28. Altmetric Badge
    Chapter 27 Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding
  29. Altmetric Badge
    Chapter 28 Isolation and Characterization of Endogenous RNPs from Brain Tissues
  30. Altmetric Badge
    Chapter 29 Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein–RNA Interactions
  31. Altmetric Badge
    Chapter 30 RNA Tagging: Preparation of High-Throughput Sequencing Libraries
  32. Altmetric Badge
    Chapter 31 RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells
  33. Altmetric Badge
    Chapter 32 Erratum to: Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Attention for Chapter 3: Identifying the m6A Methylome by Affinity Purification and Sequencing
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Above-average Attention Score compared to outputs of the same age and source (60th percentile)

Mentioned by

twitter
2 X users

Citations

dimensions_citation
10 Dimensions

Readers on

mendeley
15 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Identifying the m6A Methylome by Affinity Purification and Sequencing
Chapter number 3
Book title
RNA Detection
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7213-5_3
Pubmed ID
Book ISBNs
978-1-4939-7212-8, 978-1-4939-7213-5
Authors

Phillip J. Hsu, Chuan He

Abstract

N (6)-methyladenosine (m(6)A) is the most abundant internal modification in eukaryotic mRNA, and is newly emerging as a key posttranscriptional mRNA regulator. Recent research has uncovered insight into the location and function of m(6)A sites on a large scale, in part due to the transcriptome-wide identification of m(6)A sites by high-throughput sequencing (m(6)A-seq). Here, we present a protocol for m(6)A-seq, which maps the m(6)A methylome by affinity purification and sequencing.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 53%
Student > Bachelor 1 7%
Researcher 1 7%
Student > Master 1 7%
Unknown 4 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 40%
Agricultural and Biological Sciences 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Nursing and Health Professions 1 7%
Medicine and Dentistry 1 7%
Other 0 0%
Unknown 4 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2017.
All research outputs
#14,958,596
of 23,007,887 outputs
Outputs from Methods in molecular biology
#4,728
of 13,157 outputs
Outputs of similar age
#255,668
of 442,278 outputs
Outputs of similar age from Methods in molecular biology
#508
of 1,498 outputs
Altmetric has tracked 23,007,887 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,278 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.