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Mendeley readers
Chapter title |
Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding
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Chapter number | 27 |
Book title |
RNA Detection
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7213-5_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7212-8, 978-1-4939-7213-5
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Authors |
Miha Milek, Markus Landthaler |
Abstract |
RNA-binding proteins are dynamic posttranscriptional regulators of gene expression. Identification of mRNA-binding proteins in a given experimental setting is thus of great importance. We describe a procedure to enrich for direct poly(A)(+) RNA protein binders by 4-thiouridine-enhanced UV cross-linking and oligo(dT) purification. Subsequent nuclease-mediated release of RNA-binding proteins (RBPs) from mRNA allows for detection of eluted proteins by mass spectrometry. In addition, we provide a comparative approach to detect differences in RBP binding activity upon a biological stimulus. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 50% |
Researcher | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 75% |
Agricultural and Biological Sciences | 1 | 25% |