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RNA Detection

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Cover of 'RNA Detection'

Table of Contents

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    Book Overview
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    Chapter 1 The Secret Life of RNA: Lessons from Emerging Methodologies
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    Chapter 2 Quantification of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
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    Chapter 3 Identifying the m6A Methylome by Affinity Purification and Sequencing
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    Chapter 4 PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
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    Chapter 5 Axon-TRAP-RiboTag: Affinity Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
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    Chapter 6 LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
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    Chapter 7 Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
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    Chapter 8 Single mRNA Molecule Detection in Drosophila
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    Chapter 9 Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
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    Chapter 10 Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
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    Chapter 11 Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
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    Chapter 12 Hybridization Chain Reaction for Direct mRNA Detection Without Nucleic Acid Purification
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    Chapter 13 In Situ Detection of MicroRNA Expression with RNAscope Probes
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    Chapter 14 Padlock Probes to Detect Single Nucleotide Polymorphisms
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    Chapter 15 Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
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    Chapter 16 Optimizing Molecular Beacons for Intracellular Analysis of RNA
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    Chapter 17 Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
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    Chapter 18 In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes
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    Chapter 19 Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers
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    Chapter 20 Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe
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    Chapter 21 RNA Live Imaging in the Model Microorganism Ustilago maydis
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    Chapter 22 Real-Time Fluorescence Imaging of Single-Molecule Endogenous Noncoding RNA in Living Cells
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    Chapter 23 Live Imaging of mRNA Synthesis in Drosophila
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    Chapter 24 Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
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    Chapter 25 Detection of the First Round of Translation: The TRICK Assay
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    Chapter 26 Imaging Translation Dynamics of Single mRNA Molecules in Live Cells
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    Chapter 27 Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding
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    Chapter 28 Isolation and Characterization of Endogenous RNPs from Brain Tissues
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    Chapter 29 Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein–RNA Interactions
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    Chapter 30 RNA Tagging: Preparation of High-Throughput Sequencing Libraries
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    Chapter 31 RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells
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    Chapter 32 Erratum to: Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Attention for Chapter 30: RNA Tagging: Preparation of High-Throughput Sequencing Libraries
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Chapter title
RNA Tagging: Preparation of High-Throughput Sequencing Libraries
Chapter number 30
Book title
RNA Detection
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7213-5_30
Pubmed ID
Book ISBNs
978-1-4939-7212-8, 978-1-4939-7213-5
Authors

Christopher P. Lapointe, Marvin Wickens

Abstract

Protein-RNA networks, in which a single protein binds and controls multiple mRNAs, are central in biological control. As a result, methods to identify protein-RNA interactions that occur in vivo are valuable. The "RNA Tagging" approach enables the investigator to unambiguously identify global protein-RNA interactions in vivo and is independent of protein purification, cross-linking, and radioactive labeling steps. Here, we provide a protocol to prepare high-throughput sequencing libraries for RNA Tagging experiments.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 57%
Student > Ph. D. Student 2 29%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 2 29%
Immunology and Microbiology 1 14%
Unknown 2 29%