Chapter title |
Synergizing 13C Metabolic Flux Analysis and Metabolic Engineering for Biochemical Production
|
---|---|
Chapter number | 2 |
Book title |
Synthetic Biology – Metabolic Engineering
|
Published in |
Advances in biochemical engineering biotechnology, January 2017
|
DOI | 10.1007/10_2017_2 |
Pubmed ID | |
Book ISBNs |
978-3-31-955317-7, 978-3-31-955318-4
|
Authors |
Weihua Guo, Jiayuan Sheng, Xueyang Feng |
Abstract |
Metabolic engineering of industrial microorganisms to produce chemicals, fuels, and drugs has attracted increasing interest as it provides an environment-friendly and renewable route that does not depend on depleting petroleum sources. However, the microbial metabolism is so complex that metabolic engineering efforts often have difficulty in achieving a satisfactory yield, titer, or productivity of the target chemical. To overcome this challenge, (13)C Metabolic Flux Analysis ((13)C-MFA) has been developed to investigate rigorously the cell metabolism and quantify the carbon flux distribution in central metabolic pathways. In the past decade, (13)C-MFA has been widely used in academic labs and the biotechnology industry to pinpoint the key issues related to microbial-based chemical production and to guide the development of the appropriate metabolic engineering strategies for improving the biochemical production. In this chapter we introduce the basics of (13)C-MFA and illustrate how (13)C-MFA has been applied to synergize with metabolic engineering to identify and tackle the rate-limiting steps in biochemical production. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
China | 1 | 3% |
Unknown | 28 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 24% |
Researcher | 5 | 17% |
Professor | 3 | 10% |
Student > Master | 3 | 10% |
Student > Bachelor | 2 | 7% |
Other | 5 | 17% |
Unknown | 4 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 28% |
Agricultural and Biological Sciences | 6 | 21% |
Chemical Engineering | 2 | 7% |
Immunology and Microbiology | 2 | 7% |
Business, Management and Accounting | 1 | 3% |
Other | 3 | 10% |
Unknown | 7 | 24% |