Title |
Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats
|
---|---|
Published in |
Virology Journal, November 2014
|
DOI | 10.1186/s12985-014-0196-x |
Pubmed ID | |
Authors |
Melina Fischer, Conrad M Freuling, Thomas Müller, Juliane Schatz, Thomas Bruun Rasmussen, Mariann Chriel, Anne Balkema-Buschmann, Martin Beer, Bernd Hoffmann |
Abstract |
BackgroundIn the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N¿=¿2297) and Danish (N¿=¿134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).FindingsIn total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences.ConclusionsSeveral novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae. |
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Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
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Cameroon | 1 | 4% |
Unknown | 25 | 96% |
Demographic breakdown
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Researcher | 7 | 27% |
Student > Ph. D. Student | 5 | 19% |
Other | 4 | 15% |
Student > Master | 4 | 15% |
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Other | 1 | 4% |
Unknown | 3 | 12% |
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Mathematics | 1 | 4% |
Other | 6 | 23% |
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