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Complete sequences of 4 viral hemorrhagic septicemia virus IVb isolates and their virulence in northern pike fry

Overview of attention for article published in Diseases of Aquatic Organisms, November 2017
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Title
Complete sequences of 4 viral hemorrhagic septicemia virus IVb isolates and their virulence in northern pike fry
Published in
Diseases of Aquatic Organisms, November 2017
DOI 10.3354/dao03171
Pubmed ID
Authors

RG Getchell, ER Cornwell, S Bogdanowicz, J Andrés, WN Batts, G Kurath, R Breyta, JG Choi, JM Farrell, PR Bowser

Abstract

Four viral hemorrhagic septicemia virus (VHSV) genotype IVb isolates were sequenced, their genetic variation explored, and comparative virulence assayed with experimental infections of northern pike Esox lucius fry. In addition to the type strain MI03, the complete 11183 bp genome of the first round goby Neogobius melanostomus isolate from the St. Lawrence River, and the 2013 and 2014 isolates from gizzard shad Dorosoma cepedianum die-offs in Irondequoit Bay, Lake Ontario and Dunkirk Harbor, Lake Erie were all deep sequenced on an Illumina platform. Mutations documented in the 11 yr since the MI03 index case from Lake St. Clair muskellunge Esox masquinongy showed 87 polymorphisms among the 4 isolates. Twenty-six mutations were non-synonymous and located at 18 different positions within the matrix protein, glycoprotein, non-virion protein, and RNA polymerase genes. The same 4 isolates were used to infect northern pike fry by a single 1 h bath exposure. Cumulative percent mortality varied from 42.5 to 62.5%. VHSV was detected in 57% (41/72) of the survivors at the end of the 21-d trial, suggesting that the virus was not rapidly cleared. Lesions were observed in many of the moribund and dead northern pike, such as hemorrhaging in the skin and fins, as well as hydrocephalus. Mean viral load measured from the trunk and visceral tissues of MI03-infected pike was significantly higher than the quantities detected in fish infected with the most recent isolates of genotype IVb, but there were no differences in cumulative mortality observed.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 21%
Student > Ph. D. Student 3 16%
Other 2 11%
Student > Doctoral Student 2 11%
Professor 1 5%
Other 2 11%
Unknown 5 26%
Readers by discipline Count As %
Environmental Science 4 21%
Veterinary Science and Veterinary Medicine 2 11%
Biochemistry, Genetics and Molecular Biology 1 5%
Nursing and Health Professions 1 5%
Agricultural and Biological Sciences 1 5%
Other 2 11%
Unknown 8 42%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 November 2017.
All research outputs
#9,735,640
of 12,177,594 outputs
Outputs from Diseases of Aquatic Organisms
#548
of 725 outputs
Outputs of similar age
#240,974
of 336,535 outputs
Outputs of similar age from Diseases of Aquatic Organisms
#9
of 17 outputs
Altmetric has tracked 12,177,594 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 725 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 336,535 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.