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Synthetic Biology

Overview of attention for book
Cover of 'Synthetic Biology'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 What Is Synthetic Biology?
  3. Altmetric Badge
    Chapter 2 Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
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    Chapter 3 BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit
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    Chapter 4 Overlap Extension PCR Cloning
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    Chapter 5 One-Step Isothermal Assembly of DNA Fragments
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    Chapter 6 Creation and Characterization of Component Libraries for Synthetic Biology
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    Chapter 7 In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements
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    Chapter 8 In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli
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    Chapter 9 Construction and Engineering of Large Biochemical Pathways via DNA Assembler
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    Chapter 10 Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries Through ePathBrick Vectors
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    Chapter 11 Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research
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    Chapter 12 Combinatorial DNA Assembly Using Golden Gate Cloning
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    Chapter 13 Construction of Synthetic Gene Circuits in the Escherichia coli Genome
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    Chapter 14 Shuffling of DNA cassettes in a synthetic integron.
  16. Altmetric Badge
    Chapter 15 Systematic Methodology for the Development of Mathematical Models for Biological Processes
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    Chapter 16 SOBOLHDMR: A General-Purpose Modeling Software
Attention for Chapter 13: Construction of Synthetic Gene Circuits in the Escherichia coli Genome
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  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

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Chapter title
Construction of Synthetic Gene Circuits in the Escherichia coli Genome
Chapter number 13
Book title
Synthetic Biology
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-625-2_13
Pubmed ID
Book ISBNs
978-1-62703-624-5, 978-1-62703-625-2
Authors

Bei-Wen Ying, Yuya Akeno, Tetsuya Yomo

Abstract

The construction of stable and functional synthetic circuits in bacteria is necessary in the areas of systems and synthetic biology. The common approach using plasmids to carry foreign genetic circuits offers convenience in genetic construction but is poor in genetic stability (e.g., variation in copy number). Genome recombination provides the stable genetic maintenance of synthetic circuits, but is often labor intensive and time consuming when the genetic circuits are complex and the DNA fragments become larger. The method introduced here is modified from that reported by Wanner's group and is available for integration of complex genetic circuits into the Escherichia coli chromosome.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 23%
Researcher 4 18%
Student > Doctoral Student 3 14%
Student > Master 3 14%
Student > Postgraduate 2 9%
Other 2 9%
Unknown 3 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 41%
Biochemistry, Genetics and Molecular Biology 7 32%
Environmental Science 1 5%
Veterinary Science and Veterinary Medicine 1 5%
Chemistry 1 5%
Other 0 0%
Unknown 3 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 September 2013.
All research outputs
#14,177,097
of 22,721,584 outputs
Outputs from Methods in molecular biology
#4,163
of 13,083 outputs
Outputs of similar age
#167,547
of 280,759 outputs
Outputs of similar age from Methods in molecular biology
#156
of 341 outputs
Altmetric has tracked 22,721,584 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,083 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,759 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 341 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.