| Chapter title |
Creation and Characterization of Component Libraries for Synthetic Biology
|
|---|---|
| Chapter number | 6 |
| Book title |
Synthetic Biology
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-625-2_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-624-5, 978-1-62703-625-2
|
| Authors |
Tim Weenink, Tom Ellis |
| Abstract |
Large numbers of well-described components are essential for advanced synthetic biology and model-guided design of pathways and regulatory networks. Here a method is presented for the creation of libraries of novel control elements. From these libraries, parts with well-defined properties can be selected and used in construction of finely tuned synthetic systems. The example of the PFY1 promoter in S. cerevisiae is used to describe library creation using degenerate synthetic oligos and the circular polymerase extension cloning (CPEC) method. Additionally the workflow of screening the raw library for functional parts is included to provide a full overview of the process of creating and characterizing a component library for synthetic biology. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 33% |
| Researcher | 3 | 20% |
| Student > Bachelor | 2 | 13% |
| Student > Master | 2 | 13% |
| Other | 1 | 7% |
| Other | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 60% |
| Engineering | 2 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Chemical Engineering | 1 | 7% |
| Computer Science | 1 | 7% |
| Other | 1 | 7% |