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Synthetic Biology

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Cover of 'Synthetic Biology'

Table of Contents

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    Book Overview
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    Chapter 1 What Is Synthetic Biology?
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    Chapter 2 Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
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    Chapter 3 BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit
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    Chapter 4 Overlap Extension PCR Cloning
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    Chapter 5 One-Step Isothermal Assembly of DNA Fragments
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    Chapter 6 Creation and Characterization of Component Libraries for Synthetic Biology
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    Chapter 7 In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements
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    Chapter 8 In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli
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    Chapter 9 Construction and Engineering of Large Biochemical Pathways via DNA Assembler
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    Chapter 10 Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries Through ePathBrick Vectors
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    Chapter 11 Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research
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    Chapter 12 Combinatorial DNA Assembly Using Golden Gate Cloning
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    Chapter 13 Construction of Synthetic Gene Circuits in the Escherichia coli Genome
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    Chapter 14 Shuffling of DNA cassettes in a synthetic integron.
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    Chapter 15 Systematic Methodology for the Development of Mathematical Models for Biological Processes
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    Chapter 16 SOBOLHDMR: A General-Purpose Modeling Software
Attention for Chapter 7: In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements
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Chapter title
In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements
Chapter number 7
Book title
Synthetic Biology
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-625-2_7
Pubmed ID
Book ISBNs
978-1-62703-624-5, 978-1-62703-625-2
Authors

James Chappell, Paul Freemont

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 23%
Researcher 3 23%
Other 2 15%
Student > Master 2 15%
Student > Bachelor 1 8%
Other 0 0%
Unknown 2 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 38%
Environmental Science 1 8%
Business, Management and Accounting 1 8%
Biochemistry, Genetics and Molecular Biology 1 8%
Linguistics 1 8%
Other 1 8%
Unknown 3 23%