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Synthetic Biology

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Cover of 'Synthetic Biology'

Table of Contents

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    Book Overview
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    Chapter 1 What Is Synthetic Biology?
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    Chapter 2 Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
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    Chapter 3 BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit
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    Chapter 4 Overlap Extension PCR Cloning
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    Chapter 5 One-Step Isothermal Assembly of DNA Fragments
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    Chapter 6 Creation and Characterization of Component Libraries for Synthetic Biology
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    Chapter 7 In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements
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    Chapter 8 In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli
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    Chapter 9 Construction and Engineering of Large Biochemical Pathways via DNA Assembler
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    Chapter 10 Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries Through ePathBrick Vectors
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    Chapter 11 Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research
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    Chapter 12 Combinatorial DNA Assembly Using Golden Gate Cloning
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    Chapter 13 Construction of Synthetic Gene Circuits in the Escherichia coli Genome
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    Chapter 14 Shuffling of DNA cassettes in a synthetic integron.
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    Chapter 15 Systematic Methodology for the Development of Mathematical Models for Biological Processes
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    Chapter 16 SOBOLHDMR: A General-Purpose Modeling Software
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