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Sepsis

Overview of attention for book
Cover of 'Sepsis'

Table of Contents

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    Book Overview
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    Chapter 1 Microbiological Diagnosis of Sepsis: The Confounding Effects of a “Gold Standard”
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    Chapter 2 Pathophysiological aspects of sepsis: an overview.
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    Chapter 3 Clinical aspects of sepsis: an overview.
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    Chapter 4 Technical Improvements in Culturing Blood
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    Chapter 5 Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF/MS)-Based Identification of Pathogens from Positive Blood Culture Bottles
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    Chapter 6 Bacterial and Fungal DNA Extraction from Positive Blood Culture Bottles: A Manual and an Automated Protocol
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    Chapter 7 Broad-range PCR in the identification of bacterial and fungal pathogens from positive blood culture bottles: a sequencing approach.
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    Chapter 8 Identification of bacterial and fungal pathogens from positive blood culture bottles: a microarray-based approach.
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    Chapter 9 Detection of Carbapenemases Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay.
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    Chapter 10 Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures
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    Chapter 11 Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols
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    Chapter 12 Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols
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    Chapter 13 Broad-range PCR for the identification of bacterial and fungal pathogens from blood: a sequencing approach.
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    Chapter 14 Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples
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    Chapter 15 Host response biomarkers in the diagnosis of sepsis: a general overview.
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    Chapter 16 Host Response Biomarkers in Sepsis: The Role of Procalcitonin
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    Chapter 17 Host Response Biomarkers in Sepsis: Overview on sTREM-1 Detection.
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    Chapter 18 Host Response Biomarker in Sepsis: suPAR Detection
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    Chapter 19 Clinical Diagnosis of Sepsis and the Combined Use of Biomarkers and Culture- and Non-Culture-Based Assays
Attention for Chapter 13: Broad-range PCR for the identification of bacterial and fungal pathogens from blood: a sequencing approach.
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Chapter title
Broad-range PCR for the identification of bacterial and fungal pathogens from blood: a sequencing approach.
Chapter number 13
Book title
Sepsis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1776-1_13
Pubmed ID
Book ISBNs
978-1-4939-1775-4, 978-1-4939-1776-1
Authors

Eva Leitner, Harald H Kessler, Harald H. Kessler, Leitner, Eva, Kessler, Harald H.

Abstract

Broad-range PCR has become a valuable tool for the identification of microorganisms in the clinical laboratory over the last years. It was primarily used to identify slow-growing and fastidious microorganisms with poor biochemical activity. Nowadays, it is also used to identify microorganisms directly from clinical samples such as blood or punctuates from primarily sterile body sites. In these specimens, the usage of broad-range PCR is challenging regarding contamination and standardization. To overcome these problems, a new test system, the SepsiTest™, was introduced recently employing broad-range PCR for the identification of microorganisms in septic patients. In this chapter, the test system is described and the equipment necessary listed.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 2 22%
Researcher 2 22%
Student > Ph. D. Student 2 22%
Unspecified 1 11%
Student > Bachelor 1 11%
Other 1 11%
Readers by discipline Count As %
Medicine and Dentistry 4 44%
Agricultural and Biological Sciences 2 22%
Unspecified 1 11%
Chemistry 1 11%
Immunology and Microbiology 1 11%
Other 0 0%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2014.
All research outputs
#20,239,689
of 22,766,595 outputs
Outputs from Methods in molecular biology
#9,865
of 13,090 outputs
Outputs of similar age
#295,623
of 352,895 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,895 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.