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Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches

Overview of attention for article published in Environmental Science & Technology, November 2014
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Title
Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches
Published in
Environmental Science & Technology, November 2014
DOI 10.1021/es5038657
Pubmed ID
Authors

Ryan J. Oster, Rasanthi U. Wijesinghe, Sheridan K. Haack, Lisa R. Fogarty, Taaja R. Tucker, Stephen C. Riley

Abstract

Quantitative assessment of bacterial pathogens, their geographic variability, and distribution in various matrices at Great Lakes beaches are limited. Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 (eaeO157), shiga-toxin producing E. coli (stx2), Campylobacter jejuni (mapA), Shigella spp. (ipaH), and a Salmonella enterica-specific (SE) DNA sequence at seven Great Lakes beaches, in algae, water, and sediment. Overall, detection frequencies were mapA>stx2>ipaH>SE>eaeO157. Results were highly variable among beaches and matrices; some correlations with environmental conditions were observed for mapA, stx2, and ipaH detections. Beach seasonal mean mapA abundance in water was correlated with beach seasonal mean log10 E. coli concentration. At one beach, stx2 gene abundance was positively correlated with concurrent daily E. coli concentrations. Concentration distributions for stx2, ipaH, and mapA within algae, sediment, and water were statistically different (Non-Detect and Data Analysis in R). Assuming 10, 50, or 100% of gene copies represented viable and presumably infective cells, a quantitative microbial risk assessment tool developed by Michigan State University indicated a moderate probability of illness for Campylobacter jejuni at the study beaches, especially where recreational water quality criteria were exceeded. Pathogen gene quantification may be useful for beach water quality management.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 1%
United States 1 1%
Canada 1 1%
Unknown 64 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 25%
Researcher 13 19%
Professor > Associate Professor 7 10%
Student > Master 4 6%
Student > Bachelor 3 4%
Other 9 13%
Unknown 14 21%
Readers by discipline Count As %
Environmental Science 13 19%
Agricultural and Biological Sciences 11 16%
Engineering 9 13%
Biochemistry, Genetics and Molecular Biology 8 12%
Immunology and Microbiology 3 4%
Other 4 6%
Unknown 19 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 December 2014.
All research outputs
#22,760,732
of 25,377,790 outputs
Outputs from Environmental Science & Technology
#19,590
of 20,675 outputs
Outputs of similar age
#315,652
of 369,553 outputs
Outputs of similar age from Environmental Science & Technology
#244
of 264 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,675 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 369,553 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 264 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.