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Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus) — towards understanding gene function and regulatory evolution in an ecological model system for rapid phenotypic…

Overview of attention for article published in BMC Developmental Biology, November 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (63rd percentile)
  • Good Attention Score compared to outputs of the same age and source (76th percentile)

Mentioned by

twitter
6 tweeters
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1 Facebook page

Citations

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8 Dimensions

Readers on

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20 Mendeley
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Title
Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus) — towards understanding gene function and regulatory evolution in an ecological model system for rapid phenotypic diversification
Published in
BMC Developmental Biology, November 2017
DOI 10.1186/s12861-017-0157-x
Pubmed ID
Authors

Claudius F. Kratochwil, Maggie M. Sefton, Yipeng Liang, Axel Meyer

Abstract

The Midas cichlid species complex (Amphilophus spp.) is widely known among evolutionary biologists as a model system for sympatric speciation and adaptive phenotypic divergence within extremely short periods of time (a few hundred generations). The repeated parallel evolution of adaptive phenotypes in this radiation, combined with their near genetic identity, makes them an excellent model for studying phenotypic diversification. While many ecological and evolutionary studies have been performed on Midas cichlids, the molecular basis of specific phenotypes, particularly adaptations, and their underlying coding and cis-regulatory changes have not yet been studied thoroughly. For the first time in any New World cichlid, we use Tol2 transposon-mediated transgenesis in the Midas cichlid (Amphilophus citrinellus). By adapting existing microinjection protocols, we established an effective protocol for transgenesis in Midas cichlids. Embryos were injected with a Tol2 plasmid construct that drives enhanced green fluorescent protein (eGFP) expression under the control of the ubiquitin promoter. The transgene was successfully integrated into the germline, driving strong ubiquitous expression of eGFP in the first transgenic Midas cichlid line. Additionally, we show transient expression of two further transgenic constructs, ubiquitin::tdTomato and mitfa::eGFP. Transgenesis in Midas cichlids will facilitate further investigation of the genetic basis of species-specific traits, many of which are adaptations. Transgenesis is a versatile tool not only for studying regulatory elements such as promoters and enhancers, but also for testing gene function through overexpression of allelic gene variants. As such, it is an important first step in establishing the Midas cichlid as a powerful model for studying adaptive coding and non-coding changes in an ecological and evolutionary context.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 30%
Researcher 4 20%
Student > Bachelor 2 10%
Student > Doctoral Student 2 10%
Student > Master 2 10%
Other 2 10%
Unknown 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 45%
Biochemistry, Genetics and Molecular Biology 5 25%
Engineering 2 10%
Computer Science 1 5%
Environmental Science 1 5%
Other 0 0%
Unknown 2 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 August 2018.
All research outputs
#4,140,978
of 13,767,969 outputs
Outputs from BMC Developmental Biology
#93
of 305 outputs
Outputs of similar age
#137,976
of 395,708 outputs
Outputs of similar age from BMC Developmental Biology
#4
of 17 outputs
Altmetric has tracked 13,767,969 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 305 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 395,708 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.