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Anti-carbamylated protein antibodies precede disease onset in monkeys with collagen-induced arthritis

Overview of attention for article published in Arthritis Research & Therapy, November 2017
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Title
Anti-carbamylated protein antibodies precede disease onset in monkeys with collagen-induced arthritis
Published in
Arthritis Research & Therapy, November 2017
DOI 10.1186/s13075-017-1455-1
Pubmed ID
Authors

Marije K. Verheul, Michel P. M. Vierboom, Bert A. ’t Hart, Rene E. M. Toes, Leendert A. Trouw

Abstract

Rheumatoid factor (RF), anti-citrullinated protein antibodies (ACPA) and anti-carbamylated protein (anti-CarP) antibodies are rheumatoid arthritis (RA)-associated autoantibodies. Besides their presence in human serum, anti-CarP antibodies have also been described in rodent models of arthritis, while ACPA are not consistently detectable. Data on these RA-associated autoantibodies in primates are not available. Therefore, we investigated the presence of RF, anti-CarP antibodies and ACPA in rhesus monkeys before and after collagen-induced arthritis immunizations. In previous studies, arthritis was induced in groups of rhesus monkeys by immunisation with collagen following pre-treatment with placebo, abatacept or Roactemra. Previously collected serum was used to measure, autoantibodies by ELISA, detecting anti-CarP antibodies, RF-IgM and antibodies against CCP2, citrullinated myelin basic protein and citrullinated fibrinogen. Out of the three autoantibodies, only anti-CarP antibodies were detectable in resus monkeys with arthritis. RF-IgM and ACPA were undetectable and below the detection limit of the ELISA. The level of anti-CarP antibodies increases over time and, similar to in humans and mice, these autoantibodies were already detectable before clinical disease onset. Furthermore, preventive treatment with abatacept (CTLA4/IgG1-Fc fusion protein) inhibited the development of anti-CarP antibodies after immunization, while this was less evident for preventive Roactemra (anti-IL6-receptor) treatment. Moreover, disease progression was only reduced following abatacept treatment. Rhesus monkeys develop anti-CarP antibodies upon induction of collagen-induced arthritis, while we were unable to detect RF or ACPA. Also, the development of anti-CarP antibodies could be inhibited by preventive abatacept treatment.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 38%
Other 2 25%
Student > Postgraduate 1 13%
Professor 1 13%
Unspecified 1 13%
Other 0 0%
Readers by discipline Count As %
Medicine and Dentistry 2 25%
Unspecified 2 25%
Agricultural and Biological Sciences 2 25%
Immunology and Microbiology 1 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 13%
Other 0 0%