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Crotalus durissus terrificus crotapotin naturally displays preferred positions for amino acid substitutions

Overview of attention for article published in Journal of Venomous Animals and Toxins including Tropical Diseases, November 2017
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Title
Crotalus durissus terrificus crotapotin naturally displays preferred positions for amino acid substitutions
Published in
Journal of Venomous Animals and Toxins including Tropical Diseases, November 2017
DOI 10.1186/s40409-017-0136-5
Pubmed ID
Authors

Laudicéia Alves de Oliveira, Rui Seabra Ferreira, Benedito Barraviera, Francilene Capel Tavares de Carvalho, Luciana Curtolo de Barros, Lucilene Delazari dos Santos, Daniel Carvalho Pimenta

Abstract

Classically, Crotalus durissus terrificus (Cdt) venom can be described, according to chromatographic criteria, as a simple venom, composed of four major toxins, namely: gyroxin, crotamine, crotoxin and convulxin. Crotoxin is a non-covalent heterodimeric neurotoxin constituted of two subunits: an active phospholipase A2 and a chaperone protein, termed crotapotin. This molecule is composed of three peptide chains connected by seven disulfide bridges. Naturally occurring variants/isoforms of either crotoxin or crotapotin itself have already been reported. The crude Cdt venom was separated by using RP-HPLC and the toxins were identified by mass spectrometry (MS). Crotapotin was purified, reduced and alkylated in order to separate the peptide chains that were further analyzed by mass spectrometry and de novo peptide sequencing. The RP-HPLC profile of the isolated crotapotin chains already indicated that the α chain would present isoforms, which was corroborated by the MS and tandem mass spectrometry analyses. It was possible to observe that the Cdt crotapotin displays a preferred amino acid substitution pattern present in the α chain, at positions 31 and 40. Moreover, substitutions could also be observed in β and γ chains (one for each). The combinations of these four different peptides, with the already described chains, would produce ten different crotapotins, which is compatible to our previous observations for the Cdt venom.

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Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 42%
Unspecified 1 8%
Student > Ph. D. Student 1 8%
Researcher 1 8%
Student > Doctoral Student 1 8%
Other 0 0%
Unknown 3 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 33%
Biochemistry, Genetics and Molecular Biology 2 17%
Unspecified 1 8%
Medicine and Dentistry 1 8%
Unknown 4 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2017.
All research outputs
#9,798,232
of 15,404,988 outputs
Outputs from Journal of Venomous Animals and Toxins including Tropical Diseases
#200
of 377 outputs
Outputs of similar age
#232,640
of 409,440 outputs
Outputs of similar age from Journal of Venomous Animals and Toxins including Tropical Diseases
#19
of 40 outputs
Altmetric has tracked 15,404,988 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 377 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 409,440 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.