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A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms

Overview of attention for article published in Bioengineered, July 2014
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Mentioned by

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2 tweeters

Citations

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46 Dimensions

Readers on

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67 Mendeley
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Title
A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms
Published in
Bioengineered, July 2014
DOI 10.4161/bioe.29898
Pubmed ID
Authors

George Seghal Kiran, Anuj Nishanth Lipton, Jonathan Kennedy, Alan DW Dobson, Joseph Selvin

Abstract

A halotolerant thermostable lipase was purified and characterized from the marine bacterium Oceanobacillus sp. PUMB02. This lipase displayed a high degree of stability over a wide range of conditions including pH, salinity, and temperature. It was optimally active at 30 °C and pH 8.0 respectively and was stable at higher temperatures (50-70 °C) and alkaline pH. The molecular mass of the lipase was approximately 31 kDa based on SDS-PAGE and MALDI-ToF fingerprint analysis. Conditions for enhanced production of lipase by Oceanobacillus sp. PUMB02 were attained in response surface method-guided optimization with factors such as olive oil, sucrose, potassium chromate, and NaCl being evaluated, resulting in levels of 58.84 U/ml being achieved. The biofilm disruption potential of the PUMB02 lipase was evaluated and compared with a marine sponge metagenome derived halotolerant lipase Lpc53E1. Good biofilm disruption activity was observed with both lipases against potential food pathogens such as Bacillus cereus MTCC1272, Listeria sp. MTCC1143, Serratia sp. MTCC4822, Escherichia coli MTCC443, Pseudomonas fluorescens MTCC1748, and Vibrio parahemolyticus MTCC459. Phase contrast microscopy, scanning electron microscopy, and confocal laser scanning microscopy showed very effective disruption of pathogenic biofilms. This study reveals that marine derived hydrolytic enzymes such as lipases may have potential utility in inhibiting biofilm formation in a food processing environment and is the first report of the potential application of lipases from the genus Oceanobacillus in biofilm disruption strategies.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 67 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 15%
Student > Bachelor 9 13%
Student > Master 8 12%
Researcher 7 10%
Student > Postgraduate 5 7%
Other 13 19%
Unknown 15 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 30%
Biochemistry, Genetics and Molecular Biology 14 21%
Immunology and Microbiology 4 6%
Environmental Science 2 3%
Business, Management and Accounting 1 1%
Other 6 9%
Unknown 20 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2014.
All research outputs
#13,832,254
of 21,321,193 outputs
Outputs from Bioengineered
#263
of 1,149 outputs
Outputs of similar age
#189,934
of 344,707 outputs
Outputs of similar age from Bioengineered
#8
of 14 outputs
Altmetric has tracked 21,321,193 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,149 research outputs from this source. They receive a mean Attention Score of 1.7. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,707 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.