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Chaperones

Overview of attention for book
Cover of 'Chaperones'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
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    Chapter 2 Role of Heat Shock Factors in Stress-Induced Transcription
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    Chapter 3 Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
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    Chapter 4 Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay
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    Chapter 5 Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay
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    Chapter 6 Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors
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    Chapter 7 A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine
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    Chapter 8 Primary Colorectal Cells Culture as a Translational Research Model
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    Chapter 9 Cell Death and Survival Assays
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    Chapter 10 Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro
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    Chapter 11 Proteomic Profiling of Hsp90 Inhibitors
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    Chapter 12 Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides
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    Chapter 13 Nucleotide Exchange Factors for Hsp70 Chaperones
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    Chapter 14 Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast
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    Chapter 15 Bacterial Hsp90 ATPase Assays
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    Chapter 16 Detecting Posttranslational Modifications of Hsp90
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    Chapter 17 Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)
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    Chapter 18 A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond
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    Chapter 19 Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors
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    Chapter 20 Computational Analysis of the Chaperone Interaction Networks
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    Chapter 21 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
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    Chapter 22 Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody
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    Chapter 23 Detection and Analysis of Extracellular Hsp90 (eHsp90)
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    Chapter 24 Molecular Chaperone Receptors
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    Chapter 25 Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy
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    Chapter 26 A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells
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    Chapter 27 Hsp70: A Cancer Target Inside and Outside the Cell
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    Chapter 28 Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding
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    Chapter 29 Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors
Attention for Chapter 26: A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells
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Chapter title
A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells
Chapter number 26
Book title
Chaperones
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7477-1_26
Pubmed ID
Book ISBNs
978-1-4939-7476-4, 978-1-4939-7477-1
Authors

Desheng Weng, Stuart K. Calderwood, Jianlin Gong

Abstract

We have developed an enhanced molecular chaperone-based vaccine through rapid isolation of Hsp70 peptide complexes after the fusion of tumor and dendritic cells (Hsp70.PC-F). In this approach, the tumor antigens are introduced into the antigen processing machinery of dendritic cells through the cell fusion process and thus we can obtain antigenic tumor peptides or their intermediates that have been processed by dendritic cells. Our results show that Hsp70.PC-F has increased immunogenicity compared to preparations from tumor cells alone and therefore constitutes an improved formulation of chaperone protein-based tumor vaccine.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 29%
Professor > Associate Professor 1 14%
Librarian 1 14%
Unknown 3 43%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 14%
Immunology and Microbiology 1 14%
Neuroscience 1 14%
Engineering 1 14%
Unknown 3 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2017.
All research outputs
#15,484,498
of 23,009,818 outputs
Outputs from Methods in molecular biology
#5,388
of 13,157 outputs
Outputs of similar age
#269,723
of 442,295 outputs
Outputs of similar age from Methods in molecular biology
#596
of 1,498 outputs
Altmetric has tracked 23,009,818 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,295 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.