↓ Skip to main content

Chaperones

Overview of attention for book
Cover of 'Chaperones'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
  3. Altmetric Badge
    Chapter 2 Role of Heat Shock Factors in Stress-Induced Transcription
  4. Altmetric Badge
    Chapter 3 Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
  5. Altmetric Badge
    Chapter 4 Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay
  6. Altmetric Badge
    Chapter 5 Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay
  7. Altmetric Badge
    Chapter 6 Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors
  8. Altmetric Badge
    Chapter 7 A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine
  9. Altmetric Badge
    Chapter 8 Primary Colorectal Cells Culture as a Translational Research Model
  10. Altmetric Badge
    Chapter 9 Cell Death and Survival Assays
  11. Altmetric Badge
    Chapter 10 Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro
  12. Altmetric Badge
    Chapter 11 Proteomic Profiling of Hsp90 Inhibitors
  13. Altmetric Badge
    Chapter 12 Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides
  14. Altmetric Badge
    Chapter 13 Nucleotide Exchange Factors for Hsp70 Chaperones
  15. Altmetric Badge
    Chapter 14 Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast
  16. Altmetric Badge
    Chapter 15 Bacterial Hsp90 ATPase Assays
  17. Altmetric Badge
    Chapter 16 Detecting Posttranslational Modifications of Hsp90
  18. Altmetric Badge
    Chapter 17 Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)
  19. Altmetric Badge
    Chapter 18 A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond
  20. Altmetric Badge
    Chapter 19 Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors
  21. Altmetric Badge
    Chapter 20 Computational Analysis of the Chaperone Interaction Networks
  22. Altmetric Badge
    Chapter 21 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
  23. Altmetric Badge
    Chapter 22 Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody
  24. Altmetric Badge
    Chapter 23 Detection and Analysis of Extracellular Hsp90 (eHsp90)
  25. Altmetric Badge
    Chapter 24 Molecular Chaperone Receptors
  26. Altmetric Badge
    Chapter 25 Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy
  27. Altmetric Badge
    Chapter 26 A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells
  28. Altmetric Badge
    Chapter 27 Hsp70: A Cancer Target Inside and Outside the Cell
  29. Altmetric Badge
    Chapter 28 Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding
  30. Altmetric Badge
    Chapter 29 Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors
Attention for Chapter 18: A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

blogs
1 blog
twitter
2 tweeters

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
13 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond
Chapter number 18
Book title
Chaperones
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7477-1_18
Pubmed ID
Book ISBNs
978-1-4939-7476-4, 978-1-4939-7477-1
Authors

Benjamin J. Lang, Kristina M. Holton, Jianlin Gong, Stuart K. Calderwood

Abstract

RNA sequencing (RNA-seq) is a powerful method of transcript analysis that allows for the sequence identification and quantification of cellular transcripts. RNA-seq has many applications including differential gene expression (DE) analysis, gene fusion detection, allele-specific expression, isoform and splice variant quantification, and identification of novel genes. These applications can be used for downstream systems biology analyses such as gene ontology analysis to provide insights into cellular processes altered between biological conditions. Given the wide range of signaling pathways subject to chaperone activity as well as numerous chaperone functions in RNA metabolism, RNA-seq may provide a valuable tool for the study of chaperone proteins in biology and disease. This chapter outlines an example RNA-seq workflow to determine differentially expressed (DE) genes between two or more sample conditions and provides some considerations for RNA-seq experimental design.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 23%
Student > Bachelor 2 15%
Professor > Associate Professor 2 15%
Unspecified 2 15%
Student > Ph. D. Student 1 8%
Other 2 15%
Unknown 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 23%
Biochemistry, Genetics and Molecular Biology 3 23%
Unspecified 2 15%
Computer Science 2 15%
Social Sciences 1 8%
Other 0 0%
Unknown 2 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 April 2018.
All research outputs
#1,819,810
of 12,829,119 outputs
Outputs from Methods in molecular biology
#473
of 8,380 outputs
Outputs of similar age
#74,194
of 386,768 outputs
Outputs of similar age from Methods in molecular biology
#52
of 1,646 outputs
Altmetric has tracked 12,829,119 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,380 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 386,768 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 1,646 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.