Chapter 1 Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

Chapter 3 Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter

Chapter 4 Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay

Chapter 5 Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay

Chapter 6 Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors

Chapter 7 A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine

Chapter 8 Primary Colorectal Cells Culture as a Translational Research Model

Chapter 9 Cell Death and Survival Assays

Chapter 10 Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro

Chapter 11 Proteomic Profiling of Hsp90 Inhibitors

Chapter 12 Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides

Chapter 13 Nucleotide Exchange Factors for Hsp70 Chaperones

Chapter 14 Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast

Chapter 15 Bacterial Hsp90 ATPase Assays

Chapter 16 Detecting Posttranslational Modifications of Hsp90

Chapter 17 Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)

Chapter 18 A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond

Chapter 19 Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors

Chapter 20 Computational Analysis of the Chaperone Interaction Networks

Chapter 21 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer

Chapter 22 Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody

Chapter 23 Detection and Analysis of Extracellular Hsp90 (eHsp90)

Chapter 24 Molecular Chaperone Receptors

Chapter 25 Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy

Chapter 26 A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells

Chapter 27 Hsp70: A Cancer Target Inside and Outside the Cell

Chapter 28 Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding

Chapter 29 Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors