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Chaperones

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Cover of 'Chaperones'

Table of Contents

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    Book Overview
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    Chapter 1 Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
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    Chapter 2 Role of Heat Shock Factors in Stress-Induced Transcription
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    Chapter 3 Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
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    Chapter 4 Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay
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    Chapter 5 Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay
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    Chapter 6 Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors
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    Chapter 7 A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine
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    Chapter 8 Primary Colorectal Cells Culture as a Translational Research Model
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    Chapter 9 Cell Death and Survival Assays
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    Chapter 10 Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro
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    Chapter 11 Proteomic Profiling of Hsp90 Inhibitors
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    Chapter 12 Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides
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    Chapter 13 Nucleotide Exchange Factors for Hsp70 Chaperones
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    Chapter 14 Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast
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    Chapter 15 Bacterial Hsp90 ATPase Assays
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    Chapter 16 Detecting Posttranslational Modifications of Hsp90
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    Chapter 17 Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)
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    Chapter 18 A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond
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    Chapter 19 Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors
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    Chapter 20 Computational Analysis of the Chaperone Interaction Networks
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    Chapter 21 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
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    Chapter 22 Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody
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    Chapter 23 Detection and Analysis of Extracellular Hsp90 (eHsp90)
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    Chapter 24 Molecular Chaperone Receptors
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    Chapter 25 Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy
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    Chapter 26 A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells
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    Chapter 27 Hsp70: A Cancer Target Inside and Outside the Cell
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    Chapter 28 Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding
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    Chapter 29 Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors
Attention for Chapter 11: Proteomic Profiling of Hsp90 Inhibitors
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Chapter title
Proteomic Profiling of Hsp90 Inhibitors
Chapter number 11
Book title
Chaperones
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7477-1_11
Pubmed ID
Book ISBNs
978-1-4939-7476-4, 978-1-4939-7477-1
Authors

Sudhakar Voruganti, Jake T. Kline, Maurie J. Balch, Janet Rogers, Robert L. Matts, Steven D. Hartson

Abstract

Mass spectrometry assays demonstrate that Hsp90 inhibitors alter the expression of approximately one-quarter of the assayable proteome in mammalian cells. These changes are extraordinarily robust and reproducible, making "proteomics profiling" the gold standard for validating the effects of new Hsp90 inhibitors on cultured cells. Proteomics assays can also suggest novel hypotheses regarding drug mechanisms. To assist investigators in adopting this approach, this Chapter provides detailed protocols for conducting simple proteomics assays of Hsp90 inhibition. The protocols present a robust label-free approach that utilizes pre-fractionation of protein samples by SDS-PAGE, thereby providing reasonably good penetration into the proteome while addressing common issues with sample quality. The actual programming and operation of liquid chromatography-tandem mass spectrometers is not covered, but expectations for achievable performance are discussed, as are alternative approaches, common challenges, and software for data analysis.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 40%
Student > Ph. D. Student 1 20%
Researcher 1 20%
Other 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 60%
Agricultural and Biological Sciences 2 40%