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Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA)

Overview of attention for article published in BMC Veterinary Research, December 2014
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2 tweeters

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2 Dimensions

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195 Mendeley
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Title
Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA)
Published in
BMC Veterinary Research, December 2014
DOI 10.1186/s12917-014-0288-2
Pubmed ID
Authors

Sabrina Thomé, Carolina Lessa-Aquino, Albert Icksang Ko, Walter Lilenbaum, Marco Alberto Medeiros

Abstract

BackgroundThe microscopic agglutination test (MAT), the standard method for serological diagnosis of leptospirosis, may present limitations regarding its sensitivity. Current studies suggest that Leptospira immunoglobulin-like (Lig) proteins and LipL32 are of particular interest as serodiagnostic markers since they are present only in pathogenic species of the Leptospira genus. The purpose of this study was to identify leptospiral immunodominant proteins that are recognized by canine sera from diseased dogs.ResultsA total of 109 dogs were studied, including seroreactive dogs (MAT ¿800) and dogs with no seroreactivity detectable by MAT. Eight recombinant fragments (31¿70 kDa) of pathogenic Leptospira were tested for their use as diagnostic markers for canine leptospirosis using the Multi-antigen Print Immunoassay (MAPIA) platform: LigB [582-947aa] from L. interrogans serovar Pomona, L. interrogans serovar Copenhageni and L. kirschneri serovar Gryppotyphosa, LigB [131-649aa] from L. interrogans serovar Copenhageni, L. interrogans serovar Canicola and L. kirschneri serovar Gryppotyphosa, LigA [625-1224aa] L. interrogans serovar Copenhageni and LipL32 from L. interrogans serovar Copenhageni. The data were analyzed and ROC curves were generated. Altogether, LigB [131-649aa] L. interrogans Canicola, LigB [131-649aa] L. kirschneri Gryppotyphosa and LipL32 L. interrogans Copenhageni showed best accuracy (AUC¿=¿0.826 to 0.869), with 70% specificity and sensitivity ranging from 89% to 95%.ConclusionsThese results reinforce their potential as diagnostic candidates for the development of new methods for the serological diagnosis of canine leptospirosis.

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The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 195 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 7 4%
India 1 <1%
Unknown 187 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 48 25%
Student > Postgraduate 28 14%
Student > Doctoral Student 24 12%
Student > Bachelor 18 9%
Student > Ph. D. Student 12 6%
Other 31 16%
Unknown 34 17%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 61 31%
Agricultural and Biological Sciences 46 24%
Medicine and Dentistry 27 14%
Biochemistry, Genetics and Molecular Biology 5 3%
Nursing and Health Professions 4 2%
Other 14 7%
Unknown 38 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2014.
All research outputs
#3,343,272
of 5,036,026 outputs
Outputs from BMC Veterinary Research
#489
of 878 outputs
Outputs of similar age
#110,673
of 175,645 outputs
Outputs of similar age from BMC Veterinary Research
#23
of 43 outputs
Altmetric has tracked 5,036,026 research outputs across all sources so far. This one is in the 29th percentile – i.e., 29% of other outputs scored the same or lower than it.
So far Altmetric has tracked 878 research outputs from this source. They receive a mean Attention Score of 2.4. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 175,645 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.