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Mendeley readers
| Title |
The utilization of SiNWs/AuNPs-modified indium tin oxide (ITO) in fabrication of electrochemical DNA sensor
|
|---|---|
| Published in |
Materials Science & Engineering: C, September 2014
|
| DOI | 10.1016/j.msec.2014.09.010 |
| Pubmed ID | |
| Authors |
Jahwarhar Izuan Abdul Rashid, Nor Azah Yusof, Jaafar Abdullah, Uda Hashim, Reza Hajian |
| Abstract |
This work describes the incorporation of SiNWs/AuNPs composite as a sensing material for DNA detection on indium tin-oxide (ITO) coated glass slide. The morphology of SiNWs/AuNPs composite as the modifier layer on ITO was studied by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). The morphological studies clearly showed that SiNWs were successfully decorated with 20nm-AuNPs using self-assembly monolayer (SAM) technique. The effective surface area for SiNWs/AuNPs-modified ITO enhanced about 10 times compared with bare ITO electrode. SiNWs/AuNPs nanocomposite was further explored as a matrix for DNA probe immobilization in detection of dengue virus as a bio-sensing model to evaluate its performance in electrochemical sensors. The hybridization of complementary DNA was monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as the redox indicator. The fabricated biosensor was able to discriminate significantly complementary, non-complementary and single-base mismatch oligonucleotides. The electrochemical biosensor was sensitive to target DNA related to dengue virus in the range of 9.0-178.0ng/ml with detection limit of 3.5ng/ml. In addition, SiNWs/AuNPs-modified ITO, regenerated up to 8 times and its stability was up to 10weeks at 4°C in silica gel. |
Mendeley readers
The data shown below were compiled from readership statistics for 101 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | <1% |
| United Kingdom | 1 | <1% |
| Paraguay | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 97 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 20% |
| Student > Master | 20 | 20% |
| Student > Bachelor | 13 | 13% |
| Researcher | 11 | 11% |
| Student > Doctoral Student | 5 | 5% |
| Other | 13 | 13% |
| Unknown | 19 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 26 | 26% |
| Engineering | 12 | 12% |
| Materials Science | 8 | 8% |
| Biochemistry, Genetics and Molecular Biology | 7 | 7% |
| Agricultural and Biological Sciences | 7 | 7% |
| Other | 16 | 16% |
| Unknown | 25 | 25% |