| Chapter title |
The interaction between plant viroid-induced symptoms and RNA silencing.
|
|---|---|
| Chapter number | 22 |
| Book title |
Antiviral Resistance in Plants
|
| Published in |
Methods in molecular biology, June 2012
|
| DOI | 10.1007/978-1-61779-882-5_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-881-8, 978-1-61779-882-5
|
| Authors |
Vicente Pallas, German Martinez, Gustavo Gomez, Pallas, Vicente, Martinez, German, Gomez, Gustavo |
| Abstract |
A large body of evidence has lead to the suggestive proposal of a potential interplay between viroid-induced pathogenesis and RNA silencing regulatory mechanisms. A variety of techniques have been used to examine this interaction. This chapter outlines the use of a green fluorescent protein (GFP) sensor, containing viroid-specific sequence targets to study this phenomenon. In addition, a symptomatic transgenic line of Nicotiana benthamiana that expresses and processes Hop stunt viroid (HSVd) is used as stocks in grafting assays with the rdr6i-Nb lines, in which the RNA-dependent RNA polymerase 6 (NbRDR6) is constitutively silenced. The combination of the use of a viroid-specific GFP-sensor, the grafting assays with plants silenced for the RDR6 and the analysis of siRNAs allows to address how to monitor viroid-specific RNA silencing and how to associate this regulatory pathway with symptom expression. |
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Geographical breakdown
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|---|---|---|
| Mexico | 1 | 6% |
| Unknown | 15 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 44% |
| Student > Ph. D. Student | 3 | 19% |
| Professor | 1 | 6% |
| Student > Master | 1 | 6% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 50% |
| Biochemistry, Genetics and Molecular Biology | 3 | 19% |
| Unknown | 5 | 31% |