Title |
The adult nasopharyngeal microbiome as a determinant of pneumococcal acquisition
|
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Published in |
Microbiome, December 2014
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DOI | 10.1186/2049-2618-2-44 |
Pubmed ID | |
Authors |
Amelieke JH Cremers, Aldert L Zomer, Jenna F Gritzfeld, Gerben Ferwerda, Sacha AFT van Hijum, Daniela M Ferreira, Joshua R Shak, Keith P Klugman, Jos Boekhorst, Harro M Timmerman, Marien I de Jonge, Stephen B Gordon, Peter WM Hermans |
Abstract |
Several cohort studies have indicated associations between S. pneumoniae and other microbes in the nasopharynx. To study causal relationships between the nasopharyngeal microbiome and pneumococcal carriage, we employed an experimental human pneumococcal carriage model. Healthy adult volunteers were assessed for pneumococcal carriage by culture of nasal wash samples (NWS). Those without natural pneumococcal carriage received an intranasal pneumococcal inoculation with serotype 6B or 23F. The composition of the nasopharyngeal microbiome was longitudinally studied by 16S rDNA pyrosequencing on NWS collected before and after challenge. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 14% |
Netherlands | 1 | 14% |
Austria | 1 | 14% |
Australia | 1 | 14% |
Unknown | 3 | 43% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 6 | 86% |
Scientists | 1 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 2 | 2% |
United Kingdom | 1 | <1% |
Brazil | 1 | <1% |
Thailand | 1 | <1% |
United States | 1 | <1% |
Unknown | 127 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 33 | 25% |
Researcher | 27 | 20% |
Student > Master | 17 | 13% |
Student > Bachelor | 12 | 9% |
Other | 7 | 5% |
Other | 19 | 14% |
Unknown | 18 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 37 | 28% |
Immunology and Microbiology | 22 | 17% |
Medicine and Dentistry | 22 | 17% |
Biochemistry, Genetics and Molecular Biology | 11 | 8% |
Nursing and Health Professions | 3 | 2% |
Other | 13 | 10% |
Unknown | 25 | 19% |