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Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa

Overview of attention for article published in Genetics and Molecular Biology, November 2014
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  • Good Attention Score compared to outputs of the same age and source (77th percentile)

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Citations

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Title
Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa
Published in
Genetics and Molecular Biology, November 2014
DOI 10.1590/s1415-47572014005000017
Pubmed ID
Authors

Geruza de Oliveira Ceita, Laurival Antônio Vilas-Boas, Marcelo Santos Castilho, Marcelo Falsarella Carazzolle, Carlos Priminho Pirovani, Alessandra Selbach-Schnadelbach, Karina Peres Gramacho, Pablo Ivan Pereira Ramos, Luciana Veiga Barbosa, Gonçalo Amarante Guimarães Pereira, Aristóteles Góes-Neto

Abstract

The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 3%
Unknown 32 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 18%
Student > Master 5 15%
Student > Bachelor 4 12%
Researcher 4 12%
Professor > Associate Professor 4 12%
Other 6 18%
Unknown 4 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 36%
Biochemistry, Genetics and Molecular Biology 8 24%
Chemistry 2 6%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Chemical Engineering 1 3%
Other 2 6%
Unknown 7 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2014.
All research outputs
#14,915,133
of 25,374,647 outputs
Outputs from Genetics and Molecular Biology
#305
of 771 outputs
Outputs of similar age
#134,709
of 275,741 outputs
Outputs of similar age from Genetics and Molecular Biology
#2
of 9 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 771 research outputs from this source. They receive a mean Attention Score of 4.8. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,741 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 7 of them.